Green analysis of glyphosate and its metabolite amino methyl phosphoric acid in tea

Authors

LI Beibei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, Hubei 430075, China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
WANG Wei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, Hubei 430075, China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, ChinaFollow
JIANG Feng, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, Hubei 430075, China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
WANG Huixia, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, Hubei 430075, China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China
CHEN Li, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075, China;Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Wuhan, Hubei 430075, China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075, China

Corresponding Author(s)

汪薇（1989—），女，湖北省食品质量安全监督检验研究院高级工程师，博士。E-mail: wangwei_hbqt@163.com

Abstract

Objective： This study aimed to develop an ion chromatography-tandem mass spectrometry (IC-MS/MS) for the analysis of glyphosate and its metabolite amino methyl phosphoric acid (AMPA) residues in tea without derivatization. Methods： Tea samples were ultrasonically extracted with 20 mmol/L NaOH aqueous solution and cleaned up by dispersive solid-phase extraction (150 mg primary-secondary amine (PSA), 15 mg C₁₈, 15 mg graphitized carbon black (GCB)). The samples were separated on an ion chromatographic column AS11-HC-4 μm (2 mm×250 mm) by using an isometric elution procedure of 35 mmol/L aqueous solution of sodium hydroxide as mobile phase. A multiple reactions monitoring (MRM) model was used to obtain the sensitivity of the method, and the isotopically labeled internal standards were applied in the calibration process. Good linearities and recoveries were obtained in the validation process. Results: The correlation coefficients R² were 0.999 8 and 0.998 2, respectively. The detection limits of glyphosate and AMPA in tea were both 0.05 mg/kg, and the quantification limits were both 0.10 mg/kg. The average recoveries of glyphosate and AMPA were 61.2%～104.9% and 61.5%～83.2%, respectively with RSDs less than 20%. Conclusion: This method meets the requirements of green analytical chemistry and can be used for routine detection of glyphosate and aminomethylphosphoric acid residues in tea.

Publication Date

3-27-2024

First Page

43

Last Page

50

DOI

10.13652/j.spjx.1003.5788.2022.80556

Recommended Citation

Beibei, LI; Wei, WANG; Feng, JIANG; Huixia, WANG; and Li, CHEN (2024) "Green analysis of glyphosate and its metabolite amino methyl phosphoric acid in tea," Food and Machinery: Vol. 40: Iss. 2, Article 6.
DOI: 10.13652/j.spjx.1003.5788.2022.80556
Available at: https://www.ifoodmm.cn/journal/vol40/iss2/6

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