Analysis of uncertainty in determination of folic acid in milk powder by microplate method

Authors

LIU Ziyin, Changsha Institute for Food and Drug Control, Changsha, Hunan 410016, China
ZHUO Yiming, Changsha Institute for Food and Drug Control, Changsha, Hunan 410016, ChinaFollow
LIU Lalan, Changsha Institute for Food and Drug Control, Changsha, Hunan 410016, China
PENG Na, Changsha Institute for Food and Drug Control, Changsha, Hunan 410016, China
WU Houlu, Changsha Institute for Food and Drug Control, Changsha, Hunan 410016, China

Corresponding Author(s)

卓一鸣（1987—），男，长沙市食品药品检验所高级工程师，硕士。E-mail：715467375@qq.com

Abstract

Objective: To analyze and evaluate the uncertainty of the determination of folic acid content in milk powder by microplate method. Methods: The content of folic acid in infant formula milk powder was determined by microplate method according to GB 5009.211—2022 "National Standard for Food Safety-Determination of Folic Acid in Foods". Various experimental factors affecting the test results were analyzed and calculated according to the uncertainty evaluation methods and guidelines such as JJF 1059.1—2012, CNAS-CL01-G003:2011 and CNAS-GL05:2011, and the synthetic standard uncertainty and expanded uncertainty were obtained. Results: The expanded uncertainty U₉₅=22.08 μg/100 g（k=2）, and the folate content of the sample was （282.33±22.08） μg/100 g. The uncertainty mainly comes from the process of microplate inoculation, the fitting of standard curve and the repeatability of measurement. Conclusion: The accuracy of pipettes and microbial factors have a great impact on the experimental results, so it is necessary to ensure the quality of the instrument and strictly follow the standardized procedures to minimize the uncertainty.

Publication Date

4-30-2024

First Page

95

Last Page

99,119

DOI

10.13652/j.spjx.1003.5788.2023.80663

Recommended Citation

Ziyin, LIU; Yiming, ZHUO; Lalan, LIU; Na, PENG; and Houlu, WU (2024) "Analysis of uncertainty in determination of folic acid in milk powder by microplate method," Food and Machinery: Vol. 40: Iss. 3, Article 13.
DOI: 10.13652/j.spjx.1003.5788.2023.80663
Available at: https://www.ifoodmm.cn/journal/vol40/iss3/13

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