•  
  •  
 

Corresponding Author(s)

任圆圆（1991—），女，长江大学讲师，博士。E-mail: yuanyren@yangtzeu.edu.cn

Abstract

Objective: This study aimed to develop antioxidant products and anti-diabetes drugs of wampee. The study aimed to research the structural characteristics, anti-oxidation and hypoglycemic properties in vitro of polysaccharides from different parts of wampee. Methods: The structural properties, in vitro antioxidant and hypoglycemic activities of polysaccharides from the fruit, peel and seeds of wampee (CWP-F, CWP-P, and CWP-S) were analyzed and compared from the aspects of chemical composition, molecular weight, monosaccharide composition, appearance, DPPH free radical scavenging rate and α-amylase inhibition rate. Results: CWP-F had the highest molecular weight and was mainly composed of galactose and arabinose, CWP-P was mainly composed of galacturonic acid and arabinose, and CWP-S was mainly composed of glucose. The absorption peaks of the carboxylic acid structure were found in the Fourier transform infrared spectra of the three polysaccharides. Scanning electron microscopy results showed that the surface structure of the three polysaccharides was fluffy. CWP-P had the strongest antioxidant capacity, and its DPPH free radical scavenging rate, OH free radical scavenging rate, and total reducing power all were higher than those of CWP-F and CWP-S. CWP-P had the strongest hypoglycemic ability, followed by CWP-F, and CWP-S had no hypoglycemic ability. Conclusion: In vitro, CWP-F, CWP-P, and CWP-S exhibited varying compositions, structures, and antioxidant and hypoglycemic activity. Notably, CWP-P has shown the most robust antioxidant and hypoglycemic properties.

Publication Date

4-30-2024

First Page

156

Last Page

164

DOI

10.13652/j.spjx.1003.5788.2023.80804

References

[1] WANG S, LIU Z, ZHAO M, et al. Chitosan-wampee seed essential oil composite film combined with cold plasma for refrigerated storage with modified atmosphere packaging: A promising technology for quality preservation of golden pompano fillets[J].International Journal of Biological Macromolecules, 2023, 224: 1 266-1 275.
[2] CHANG X, YE Y, PAN J, et al. Comparative assessment of phytochemical profiles and antioxidant activities in selected five varieties of wampee （Clausena lansium） fruits[J]. International Journal of Food Science & Technology, 2018, 53（12）: 2 680-2 686.
[3] 冯莉, 余洪清, 黄少华, 等. 无核黄皮病虫害的发生及综合防治[J].广东农业科学, 2004（6）: 63-65. FENG L, YU H Q, HUANG S H, et al. Occurrence and comprehensive control of seedless Clausena lansium pests and diseases[J]. Guangdong Agricultural Sciences, 2004（6）: 63-65.
[4] WU H, MIN T, LI X, et al. Physicochemical properties and antioxidant activities of acidic polysaccharides from wampee seeds[J]. International Journal of Biological Macromolecules, 2013, 59: 90-95.
[5] SONG C, HUANG F, LIU L, et al. Characterization and prebiotic properties of pectin polysaccharide from Clausena lansium （Lour.） Skeels fruit[J]. International Journal of Biological Macromolecules, 2022, 194: 412-421.
[6] 陈湑慧. 生姜茎叶多糖的结构解析、体外消化酵解和保肝活性研究[D]. 重庆: 西南大学, 2022: 4. CHEN X H. Structural analysis, in vitro digestion and fermentation, and hepatoprotective activity of polysaccharides from ginger stems and leaves[D]. Chongqing: Southwest University, 2022: 4.
[7] QIONG L, JUN L, JUN Y, et al. The effect of Laminaria japonica polysaccharides on the recovery of the male rat reproductive system and mating function damaged by multiple mini-doses of ionizing radiations[J]. Environmental Toxicology and Pharmacology, 2011, 31（2）: 286-294.
[8] ZHANG L, QIAO H Y, LIU H X, et al. Antioxidant, hypoglycemic and protection of acute liver injury activities of Ganoderma lucidum spore water extract[J]. Journal of Functional Foods, 2022, 97: 105254.
[9] JIA R B, LI Z R, WU J, et al. Physicochemical properties of polysaccharide fractions from Sargassum fusiforme and their hypoglycemic and hypolipidemic activities in type 2 diabetic rats[J]. International Journal of Biological Macromolecules, 2020, 147: 428-438.
[10] JAHAN S, NESA M, HOSSAIN M E, et al. In vivo and in silico evaluation of analgesic and hypoglycemic activities of Amaranthus blitum L.[J]. South African Journal of Botany, 2022, 150: 565-575.
[11] MA W, XIAO L, LIU H, et al. Hypoglycemic natural products with in vivo activities and their mechanisms: A review[J]. Food Science and Human Wellness, 2022, 11（5）: 1 087-1 100.
[12] WU H, LI M, YANG X, et al.Extraction optimization, physicochemical properties and antioxidant and hypoglycemic activities of polysaccharides from roxburgh rose （Rosa roxburghii Tratt.） leaves[J]. International Journal of Biological Macromolecules, 2020, 165: 517-529.
[13] 赵凯迪, 王秋丹, 林长青, 等. 桔梗多糖抗氧化特性及对2型糖尿病大鼠降血糖作用[J]. 食品与机械, 2022, 38（7）: 186-190, 198. ZHAO K D, WANG Q D, LIN C Q, et al. Antioxidant properties of polysaccharides from platycodon grandiflorum and its hypoglycemic effect on type 2 diabetic rats[J]. Food & Machinery, 2022, 38（7）: 186-190, 198.
[14] MASUKO T, MINAMI A, IWASAKI N, et al. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format[J]. Analytical Biochemistry, 2005, 339（1）: 69-72.
[15] GONALVE C, RODRIGUEZ-JASS R M, GOMES N, et al.Adaptation of dinitrosalicylic acid method to microtiter plates[J]. Analytical Methods, 2010, 2（12）: 2 046-2 048.
[16] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72（1/2）: 248-254.
[17] 杜妹玲. 芍药不同部位多糖提取、体外活性研究及其花草茶研制[D]. 哈尔滨: 东北农业大学, 2021: 19. DU M L. Extraction and in vitro activity analysis of polysaccharides from different parts of paeonia lactiflora pall., and development of herbal tea[D]. Harbin: Northeast Agricultural University, 2021: 19.
[18] 张嘉园. 荨麻多糖的分离纯化、结构鉴定及降糖活性研究[D]. 西安: 陕西科技大学, 2021: 27. ZHANG J Y. Study on separation, purification, structure identification and hypoglycemic activity of polysaccharides from urtica fissa E. pritz[D]. Xi'an: Shaanxi University of Science & Technology, 2021: 27.
[19] YUE Q, WANG Z, YU F, et al. Changes in metabolite profiles and antioxidant and hypoglycemic activities of Laminaria japonica after fermentation[J]. LWT-Food Science and Technology, 2022, 158: 113122.
[20] SHANG H, ZHAO J, GUO Y, et al. Extraction, purification, emulsifying property, hypoglycemic activity, and antioxidant activity of polysaccharides from comfrey[J]. Industrial Crops & Products, 2020, 146: 112183.
[21] DENG Q, WANG W, ZHANG Q, et al. Extraction optimization of polysaccharides from Gougunao tea and assessment of the antioxidant and hypoglycemic activities of its fractions in vitro[J]. Bioactive Carbohydrates and Dietary Fibre, 2021, 26: 100287.
[22] 周一鸣, 马思佳, 蒋晴怡, 等. 苦荞中芦丁和槲皮素对淀粉消化酶的抑制能力[J]. 食品科学, 2022, 43（18）: 30-37. ZHOU Y M, MA S J, JIANG Q Y, et al. Inhibition of starch-digesting enzymes by rutin and quercetin in tartary buckwheat[J]. Food Science, 2022, 43（18）: 30-37.
[23] LI S, LIU M, CHEN Z, et al. Cross-linking treatment of arabinoxylan improves its antioxidant and hypoglycemic activities after simulated in vitro digestion[J]. LWT-Food Science and Technology, 2021, 145: 111386.
[24] 刘功继. 酸碱处理对热加工莲藕片品质和细胞壁多糖结构的影响研究[D]. 武汉: 华中农业大学, 2021: 35. LIU G J. Effects of acid and alkali treatment on quality and cell wall polysaccharide of thermal processed lotus rhizomes slices[D]. Wuhan: Huazhong Agricultural University, 2021: 35.
[25] ZHU J, CHEN Z, CHEN L, et al. Comparison and structural characterization of polysaccharides from natural and artificial Se-enriched green tea[J]. International Journal of Biological Macromolecules, 2019, 130: 388-398.
[26] CHEN J, ZHOU M, LIU M, et al. Physicochemical, rheological properties and in vitro hypoglycemic activities of polysaccharide fractions from peach gum[J]. Carbohydrate Polymers, 2022, 296: 119954.
[27] HSU W K, HSU T H, LIN F Y, et al. Separation, purification, and α-glucosidase inhibition of polysaccharides from Coriolus versicolor LH1 mycelia[J]. Carbohydrate Polymers, 2013, 92（1）: 297-306.
[28] WANG Y, SHEN X, YIN K, et al. Structural characteristics and immune-enhancing activity of fractionated polysaccharides from Athyrium Multidentatum （Doll.） Ching[J]. International Journal of Biological Macromolecules, 2022, 205: 76-89.
[29] LU J, HE R, SUN P, et al. Molecular mechanisms of bioactive polysaccharides from Ganoderma lucidum （Lingzhi）, a review[J]. International Journal of Biological Macromolecules, 2020, 150: 765-774.
[30] WANG C C, CHANG S C, INBARAJ B S, et al. Isolation of carotenoids, flavonoids and polysaccharides from Lycium barbarum L. and evaluation of antioxidant activity[J]. Food Chemistry, 2010, 120（1）: 184-192.
[31] KIM Y M, JEONG Y K, WANG M H, et al. Inhibitory effect of pine extract on α-glucosidase activity and postprandial hyperglycemia[J]. Nutrition, 2005, 21（6）: 756-761.
[32] FU Y, FENG K L, WEI S Y, et al. Comparison of structural characteristics and bioactivities of polysaccharides from loquat leaves prepared by different drying techniques[J].International Journal of Biological Macromolecules, 2020, 145: 611-619.
[33] JIAO Y, HUA D, HUANG D, et al. Characterization of a new heteropolysaccharide from green guava and its application as an α-glucosidase inhibitor for the treatment of type II diabetes[J]. Food & Function, 2018, 9（7）: 3 997-4 007.
[34] ZHANG L, YANG J, CHEN X Q, et al. Antidiabetic and antioxidant effects of extracts from Potentilla discolor Bunge on diabetic rats induced by high fat diet and streptozotocin[J]. Journal of Ethnopharmacology, 2010, 132（2）: 518-524.
[35] CHUNG J O, YOO S H, LEE Y E, et al. Hypoglycemic potential of whole green tea: Water-soluble green tea polysaccharides combined with green tea extract delays digestibility and intestinal glucose transport of rice starch[J]. Food & Function, 2019, 10（2）: 746-753.
[36] 郑丽婷, 周鸿, 刘奕明, 等. 黄柏碱对α-葡萄糖苷酶的体外抑制作用[J]. 南京中医药大学学报, 2020, 36（6）: 853-858. ZHENG L T, ZHOU H, LIU Y M, et al. Inhibitory effect of phellodendrine on α-glucosidase in vitro[J]. Journal of Nanjing University of Traditional Chinese Medicine, 2020, 36（6）: 853-858.
[37] 姜丽丽, 张中民, 陈道玉, 等. 白藜芦醇对α-葡萄糖苷酶的抑制动力学及抑制机制[J]. 食品科学, 2019, 40（11）: 70-74. JIANG L L, ZHANG Z M, CHEN D Y, et al. Inhibition kinetics and mechanisms of resveratrol on α-glucosidase[J]. Food Science, 2019, 40（11）: 70-74.

Download

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.