Abstract
Objective: This study aimed to determine the extraction solvent and compare the distribution of functional ingredient and antioxidant activities in different parts of Penthorum chinense pursh. Methods: the total phenolic content, total flavonoid content, antioxidant capacity and individual phenolic compounds content in Penthorum chinense pursh were determined and analyzed. Results: The total phenolic content, total flavonoid content increased and then decreased with the increase of solvent concentration. The seven main phenolic compounds (catechin, rutin, pinocembrin-7-O-glucoside, quercetin, kaempferol, pinocembrin and thonningianin A), ABTS radical scavenging ability, and ferric ion reduction ability were the highest in 80% ethanol extract. The contents of total phenolic content, total flavonoid content, ABTS radical scavenging ability, DPPH radical scavenging ability and ferric ion reducing ability of different parts of Penthorum chinense pursh were flower > leaves > stem. The total phenolic content, total flavonoid content, antioxidant capacity of flower and stem decreased significantly with the increasing of storage time, while leaves were relatively stable. In addition, the antioxidant activities of Penthorum chinense pursh were significantly positively correlated with total phenolic content, catechin, isoquercetin, kaempferol-3-O-rutinoside, afzelin, and pinocembrin-7-O-glucoside (P < 0.01). Conclusion: 80% ethanol appears the most effective for polyphenol extraction in Penthorum chinense pursh, and the leaves have the great potential for development and utilization with high content and stable antioxidant activity.
Publication Date
4-30-2024
First Page
165
Last Page
172
DOI
10.13652/j.spjx.1003.5788.2023.80696
Recommended Citation
Zhuoya, XIANG; Boyu, ZHU; Yongqing, ZHU; Chen, XIA; and Song, CHEN
(2024)
"Extraction of active ingredients from Penthorum chinense pursh and comparison of component distribution in different parts,"
Food and Machinery: Vol. 40:
Iss.
3, Article 23.
DOI: 10.13652/j.spjx.1003.5788.2023.80696
Available at:
https://www.ifoodmm.cn/journal/vol40/iss3/23
References
[1] 王萌, 吴霞, 江云, 等. 赶黄草的研究进展[J]. 食品与药品, 2013, 15(3): 202-205.
WANG M, WU X, JIANG Y, et al. Progress on Penthorum chinense pursh[J]. Food and Drug, 2013, 15(3): 202-205.
[2] HE L B, ZHANG S Y, LUO C M, et al. Functional teas from the stems of Penthorum chinense pursh: Phenolic constituents, antioxidant and hepatoprotective activity[J]. Plant Foods for Human Nutrition, 2019, 74: 83-90.
[3] WANG A Q, LI M M, HUANG H M, et al. A review of Penthorum chinense pursh for hepatoprotection: Traditional use, phytochemistry, pharmacology, toxicology and clinical trials[J]. Journal of Ethnopharmacology, 2020, 251: 112569.
[4] LU Q, JIANG M H, JIANG J G, et al. Isolation and identification of compounds from Penthorum chinense pursh with antioxidant and antihepatocarcinoma properties[J]. Journal of Agricultural and Food Chemistry, 2012, 60(44): 11 097-11 103.
[5] HUANG D D, JIANG Y, CHEN W S, et al. Polyphenols with anti-proliferative activities from Penthorum chinense pursh[J]. Molecules, 2014, 19(8): 11 045-11 055.
[6] 郭艳, 兰真, 曾凡骏, 等. 赶黄草的安全性毒理学评价研究[J]. 预防医学情报杂志, 2016, 32(5): 476-481.
GUO Y, LAN Z, ZENG J F, et al. Toxicological assessment on safety of Penthorum chinense pursh[J]. Journal of Preventive Medical Information, 2016, 32(5): 476-481.
[7] 张凤梅, 卢馨, 张月天, 等. 古蔺县赶黄草食用比例人群调查研究[J]. 食品工业, 2016(11): 232-236.
ZHANG F M, LU X, ZHANG Y T, et al. Research on edible proportion of Penthorum chinense pursh through population survey in Gulin[J]. Food Industry, 2016(11): 232-236.
[8] 唐勇, 向小红, 李国春, 等. 赶黄草不同浓度乙醇提取物中没食子酸含量的测定[J]. 泸州医学院学报, 2016, 39(6): 575-578.
TANG Y, XIANG X H, LI G C, et al. Determination of the content of gallic acid in ethanol extract from different concentrations of ethanol extracts from Phethorum Chinese Pursh[J]. Journal of Luzhou Medical College, 2016, 39(6): 575-578.
[9] 陀扬凌, 金玲, 张旭. 赶黄草花与茎、叶HPLC指纹图谱比较[J]. 中国实验方剂学杂志, 2015, 21(15): 61-64.
TUO Y L, JIN L, ZHANG X. Comparison of HPLC fingerprint analysis of flos, caulis and folium from Penthor chinense[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2015, 21(15): 61-64.
[10] GUO W W, JIANG Y, CHEN X Q, et al. Identification and quantitation of major phenolic compounds from Penthorum chinense pursh by HPLC with tandem mass spectrometry and HPLC with diode array detection[J]. Journal of Separation Science, 2015, 38(16): 2 789-2 796.
[11] XIANG Z Y, XIA C, FENG S L, et al. Assessment of free and bound phenolics in the flowers and floral organs of two Camellia species flower and their antioxidant activities[J]. Food Bioscience, 2022, 49: 101905.
[12] ZHANG R, CHENG L, ZHANG T, et al. Brick tea consumption is a risk factor for dental caries and dental fluorosis among 12-year-old Tibetan children inGanzi[J]. Environ Geochem Health, 2019, 41(3): 1 405-1 417.
[13] MA Y L, SUN P, FENG J, et al. Solvent effect on phenolics and antioxidant activity of Huangshan Gongju (Dendranthema morifolium (Ramat) Tzvel. cv. Gongju) extract[J]. Food and Chemical Toxicology, 2021, 147: 111875.
[14] DENG J L, XIANG Z Y, LIN C B, et al. Identification and quantification of free, esterified, and insoluble-boundphenolics in grains of hulless barley varieties and their antioxidant activities[J]. LWT-Food Science and Technology, 2021, 151: 112001.
[15] TIAN J, CHEN J, LU F, et al. Domestic cooking methods affect the phytochemical composition and antioxidant activity of purplefleshed potatoes[J]. Food Chemistry, 2016, 197: 1 264-1 270.
[16] 朱柏雨, 夏陈, 罗棵濒, 等. 四川黑茶多酚和咖啡碱含量及其抗氧化活性分析[J]. 食品与机械, 2021, 37(8): 25-32.
ZHU B Y, XIA C, LUO K B, et al. Analysis of tea polyphenols and caffeine contents and their antioxidant activities of Sichuan dark tea[J]. Food & Machinery, 2021, 37(8): 25-32.
[17] O'SULLIVAN A, O'CALLAGHAN Y, O'GRADY M, et al. The effect of solvents on the antioxidant activity in Caco-2 cells of Irish brown seaweed extracts prepared using accelerated solvent extraction (ASE)[J]. Journal of Function Foods, 2013, 5: 940-948.
[18] DUAN H X, ZHOU C E, LI J H. Comparison of tea water extraction and ethanol-extraction extracting tea polyphenols from unfermented Pu-erh[J]. Southwest China Journal of Agricultural Science, 2013, 26(6): 2 503-2 508.
[19] 赵大洲, 石蓓梅, 叶主虹. 茶多酚水提与醇提的比较研究[J]. 食品研究与开发, 2007, 28(8): 69-71.
ZHAO D Z, SHI B M, YE Z H. A comparison study on water extraction and alcohol-extraction of tea polyphenols[J]. Food Research and Development, 2007, 28(8): 69-71.
[20] MARCANO D, HASEGAWA M. Fitoquímica orgánica[M]. Venezuela: Editorial Torino, 2002: 100.
[21] ISMAIL B B, PU Y F, GUO M M, et al. HLC-MS/QTOF identification of phytochemicals and the effects of solvents on phenolic constituents and antioxidant activity of baobab (Adansonia digitata) fruit pulp[J]. Food Chemistry, 2019, 277: 279-288.
[22] LU Q, JIANG M H, JIANG J G, et al. Isolation and identification of compounds from Penthorum chinense pursh with antioxidant and antihepatocarcinoma properties[J]. Journal of Agricultural and Food Chemistry, 2012, 60: 11 097-11 103.
[23] HE L B, ZHANG S Y, LUO C M, et al. Functional teas from the stems of Penthorum chinense pursh: Phenolic constituents, antioxidant and hepatoprotective activity[J]. Plant Foods for Human Nutrition, 2019, 74: 83-90.
[24] 范玲, 谢星星, 陈立, 等. 赶黄草3种提取物对小鼠内毒素性肝损伤的保护作用[J]. 中成药, 2019, 41(2): 291-297.
FAN L, XIE X X, CHEN L, et al. Protective effects of three Penthorum chinense extracts on endotoxin-induced liver injury in mice[J]. Chinese Traditional Patent Medicine, 2019, 41(2): 291-297.
[25] 李杰, 蒋志涛, 刘晓燕, 等. 赶黄草药理作用及其质量标志物研究进展[J]. 南京中医药大学学报, 2019, 35(6): 757-760.
LI J, JIANG Z G, LIU X Y, et al.Advances in pharmacological effect and Q-marker research of Penthorum chinese[J]. Journal of Nanjing University of Chinese Medicine, 2019, 35(6): 757-760.
[26] 向卓亚, 夏陈, 朱永清, 等. 贮藏时间对雅安藏茶中活性成分及其抗氧化活性的影响[J]. 食品工业科技, 2021, 42(11): 67-72.
XIANG Z Y, XIA C, ZHU Y Q, et al. Effect of storage time on functional ingredient and antioxidant activity of Ya'an Tibetan tea[J]. Science and Technology of Food Industry, 2021, 42(11): 67-72.
[27] 孙佩, 童文, 杨晓, 等. 赶黄草不同生育时期植株重量及有效成分含量变化研究[J]. 西南农业学报, 2013, 26(6): 2 666-2 668.
SUN P, TONG W, YANG X, et al. Study on variation of plant weight and active component contents in Herba penthori at different growth stages[J]. Southwest China Journal of Agricultural Sciences, 2013, 26(6): 2 666-2 668.
[28] SUN Z L, ZHANG Y Z, FENG Z, et al. Quality assessment of Penthorum chinense pursh through multicomponent qualification and fingerprint, chemometric, and antihepatocarcinoma analyses[J]. Food & Function, 2018, 9(7): 3 807-3 814.
[29] YIN J H, REN W, WEI B, et al. Characterization of chemical composition and prebiotic effect of a dietary medicinal plant Penthorum chinense pursh[J]. Food Chemistry, 2020, 319: 126568.
[30] 周滢, 费曜. 川产赶黄草不同部位的槲皮素含量比较[J]. 世界科学技术(中医药现代化), 2014, 16(9): 2 047-2 049.
ZHOU Y, FEI Y. Content comparison on quercetin from different parts of Penthorum chinense pursh from sichuan province[J]. World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica, 2014, 16(9): 2 047-2 049.
[31] LIU F, NG T B. Antioxidative and free radical scavenging activities of selected medicinal herbs[J]. Life Science, 2000, 66: 725-735.