Abstract
Objective: The difference in bioactive substance content and bioactivity in black beans were compared. Methods: The contents of total phenolic acids, total flavonoids, condensed tannins, total anthocyanins, total polysaccharides and total proteins of nine kinds of black beans were determined. The antioxidant activities of black bean extracts in vitro and their inhibitory effects on α-amylase, pancreatic lipase, acetylcholinesterase and tyrosinase were compared. Results: The contents of total phenolic acid, total flavonoids, condensed tannins and total anthocyanins were as follows: equine bean > small black bean > black bean; Total polysaccharide content: small black bean > black bean > horse bean; Total protein content: horse bean > black bean > small black bean. Antioxidant activity: horse bean > small black bean > black bean. In vitro experiments showed that little black bean and black bean had inhibitory effects on many enzymes, and the intensity was different. Conclusion: The contents and functions of bioactive substances in different varieties of black beans are different. Black beans are more suitable for eating, and horse bean and small black beans have higher medicinal value.
Publication Date
4-30-2024
First Page
181
Last Page
187,195
DOI
10.13652/j.spjx.1003.5788.2023.80044
Recommended Citation
Yuting, DANG; Yan, ZHANG; Boxing, JING; Xiaomeng, SU; and Xiyan, CHAI
(2024)
"Comparative study on several black bean components, antioxidant and enzyme inhibition in vitro,"
Food and Machinery: Vol. 40:
Iss.
3, Article 25.
DOI: 10.13652/j.spjx.1003.5788.2023.80044
Available at:
https://www.ifoodmm.cn/journal/vol40/iss3/25
References
[1] 张宽朝, 李敏, 汪炜姿, 等. 近20年国内外黑豆研究的文献计量研究: 基于时空发展态势的分析[J]. 金陵科技学院学报, 2020, 36（4）: 81-86.
ZHANG K C, LI M, WANG W Z, et al. Bibliometric study on Chinese and international black soybean research in the past 20 years: Analysis of space-time development status[J]. Journal of Jinling Institute of Technology, 2020, 36（4）: 81-86.
[2] 国家药典委员会. 中华人民共和国药典: 二部[S]. 北京: 中国医药科技出版社, 2020: 1 010.
Chinese Pharmacopoeia Commission. Chinese pharmacopoeia: Part II[S]. Beijing: China Medical Science and Technology Press, 2020: 1 010.
[3] 刘江, 吴海军, 陈兴福. 关于《中国药典》中黑豆鉴别方法的商榷与质量评价标准建议[J]. 中国药品标准, 2021, 22（5）: 418-423.
LIU J, WU H J, CHEN X F. Discussion on identification method and quality evaluation standard oftraditional Chinese medicine "Heidou" in the Chinese pharmacopoeia[J]. Drug Standards of China, 2021, 22（5）: 418-423.
[4] 林王敏, 翁倩倩, 邓爱平, 等. 黑豆的本草考证[J]. 中国中药杂志, 2020, 45（18）: 4 519-4 527.
LIN W M, WENG Q Q, DENG A P, et al. Textual research on sojae semen nigrum[J]. Chinese Journal of Traditional Chinese Medicine, 2020, 45（18）: 4 519-4 527.
[5] 高晶晶, 慕苗. 陕北小粒黑豆中黄酮的提取工艺研究[J]. 河南科学, 2018, 36（9）: 1 367-1 371.
GAO J J, MU M. Extraction of flavonoids from black beans in northern Shaanxi[J]. Henan Science, 2018, 36（9）: 1 367-1 371.
[6] 王双侠, 苏适, 柴宝丽. 黑豆中大豆异黄酮超声波法提取工艺优化[J]. 齐齐哈尔大学学报（自然科学版）, 2021, 37（2）: 70-72.
WANG S X, SU S, CHAI B L. Optimization of ultrasonic extraction on soy isoflavones from back beans[J]. Journal of Qiqihar University （Natural Science Edition） , 2021, 37（2）: 70-72.
[7] 孙长波, 刘宏群. 黑豆中总皂苷提取工艺的研究[J]. 食品研究与开发, 2020, 41（24）: 137-141.
SUN C B, LIU H Q. Study on extraction technology of total saponins from black bean[J]. Food Research and Development, 2020, 41（24）: 137-141.
[8] 魏玉, 王彬, 石延榜, 等. 多指标综合评分法优选中药炮制辅料黑豆汁的制备工艺[J]. 时珍国医国药, 2023, 34（3）: 605-608.
WEI Y, WANG B, SHI Y B, et al. Optimization of preparation technology of black soybean juice by multi-index comprehensive evaluation methd[J]. Lishizhen Medicine and Materia Medica Research, 2023, 34（3）: 605-608.
[9] 李佳荣, 刘美, 邓莎, 等. 不同产地黑豆质量评价[J]. 中成药, 2022, 44（8）: 2 554-2 559.
LI J R, LIU M, DENG S, et al. Quality assessment forsojae semen nigrum from different habitats[J]. Chinese Traditional Patent Medicine, 2022, 44（8）: 2 554-2 559.
[10] 李瑞, 白伟, 梁鸡保, 等. 优质专用小粒黑豆品种选育及其豆制品表现研究[J]. 大豆科技, 2022（6）: 6-14.
LI R, BAI W, LIANG J B, et al. Breeding of high quality specialized small grain black bean varieties and study on the performance of soybean products[J]. Soybean Science & Technology, 2022（6）: 6-14.
[11] 余雨婷, 张彦, 张迎, 等. 紫苏体外对代谢综合征相关四种酶抑制作用的研究[J]. 中国食品添加剂, 2022, 33（4）: 63-71.
YU Y T, ZHANG Y, ZHANG Y, et al. Study on enzyme inhibition of perilla in vitro on metabolic syndrome[J]. Chinese Food Additives, 2022, 33（4）: 63-71.
[12] 张榕欣, 邱英莲, 邓金兰, 等. 超声波辅助双酶法制备黑豆多肽的工艺优化及其抗氧化活性研究[J]. 饲料研究, 2022, 45（13）: 79-82.
ZHANG R X, QIU Y L, DENG J L, et al. Ultrasound-assisted double-enzyme method for preparing process optimization of black soybean peptides and study on its antioxidant activity[J]. Feed Research, 2022, 45（13）: 79-82.
[13] 中国农业百科全书总编辑委员会. 中国农业百科全书农作物卷: 下册[M]. 北京: 农业出版社, 1991: 824.
China Agricultural Encyclopedia Editorial Committee. Chinese agricultural encyclopedia crop volume: Volume II[M]. Beijing: Agricultural Publishing House, 1991: 824.
[14] 李思荻. 高抗氧化能力豆类的筛选及其黄酮类物质消化吸收效果的评价[D]. 大庆: 黑龙江八一农垦大学, 2010: 17.
LI S D. Bean with high antioxidant flavonoids in the seletction and evaluation of effects of digestion and absorption[D]. Daqing: Heilongjiang Bayi Agricultural University, 2010: 17.
[15] BURNS R E. Method for estimation of tannin in grain sorghum[J]. Agronomy Journal, 1971, 63: 511-512.
[16] WANG Y W, LUAN G X, ZHOU W, et al. Subcritical water extraction, UPLC-Triple-TOF/MS analysis and antioxidant activity of anthocyanins from lycium ruthenicum murr[J]. Food Chemistry, 2018, 249: 119-126.
[17] 张小荣, 黄钰芳, 何海, 等. 差示苯酚—硫酸法结合DNS法测定红芪多糖（HPS）含量[J]. 安徽农业科学, 2022, 50（4）: 186-189, 253.
ZHANG X R, HUANG Y F, HE H, et al. Differential phenol-sulfuric acid method combined with DNS method to determine the content of hedysarum polysaccharides （HPS） [J]. Journal of Anhui Agricultural Sciences, 2022, 50（4）: 186-189, 253.
[18] 王宁, 张叶韬, 芦晓芳. 黑豆异黄酮的提取分离及其对DPPH自由基的清除能力[J]. 安徽农业科学, 2022, 50（1）: 171-176.
WANG N, ZHANG Y T, LU X F. Extraction of isoflavones from Glycine max and its ability to scavenge DPPH free radicals[J]. Journal of Anhui Agricultural Sciences, 2022, 50（1）: 171-176.
[19] LIANG L F, WANG T, CAI Y S, et al. Brominated polyunsaturated lipids from the Chinese sponge Xestospongia testudinaria as a new class of pancreatic lipase inhibitors[J]. European Journal of Medicinal Chemistry, 2014, 79（8）: 290-297.
[20] 田丝竹, 李绪文, 臧爽, 等. 10种野生植物果实对α-淀粉酶和酪氨酸酶的抑制作用及其酚类化合物含量和抗氧化活性研究[J]. 分析化学, 2021, 49（3）: 449-459.
TIAN S Z, LI X W, ZANG S, et al. Investigation of α-amylase and tyrosinase inhibitory activities, phenolic compounds, and antioxidant activity in ten kinds of wild fruits[J]. Chinese Journal of Analytical Chemistry, 2021, 49（3）: 449-459.
[21] 丁林玲, 谢颖欣, 高伟, 等. 三叶木通果肉提取物的体外抗氧化及抑制α-葡萄糖苷酶和乙酰胆碱酯酶能力[J]. 南方农业学报, 2021, 52（4）: 1 058-1 065.
DING L L, XIE Y X, GAO W, et al. In vitro antioxidant activities, α-glucosidase and acetylcholinesterase inhibition ability of Akebia trifoliata pulp extracts[J]. Journal of Southern Agriculture, 2021, 52（4）: 1 058-1 065.
[22] 黄淑安, 丁红霞, 杨远帆, 等. 琯溪蜜柚疏果黄酮酶法辅助提取工艺优化及其抑制酪氨酸酶活性[J/OL]. 食品工业科技. （2023-11-03） [2023-11-22]. https://doi.org/10.13386/j.issn1002-0306.2023060298.
HUANG S A, DING H X, YANG Y F, et al. Enzyme-assisted extraction of flavonoids from Guanxi Pomelo fruit and its inhibition of tyrosinase activity[J/OL]. Food Industry Science and Technology. （2023-11-03） [2023-11-22]. https://doi.org/10.13386/j.issn1002-0306.2023060298.
[23] 余雨婷, 张彦, 张迎, 等. 基于分子对接探究紫苏粗提物对代谢综合征相关酶的抑制作用[J]. 食品与机械, 2022, 38（4）: 183-188.
YU Y T, ZHANG Y, ZHANG Y, et al. Explore the inhibitory effect of Perilla crude extract on metabolic syndrome-related enzymes based on molecular docking[J]. Food & Machinery, 2022, 38（4）: 183-188.