Abstract
Objective: To achieve rapid, quantitative and high-throughput detection of aminoglycoside antibiotic residues in pork. Methods: Square-array aligned SERS substrates based on Au nanoflowers （AuNFs） were prepared using PP synthetic paper as a substrate. Pork samples were pre-treated by QuEChERs method and subjected to SRES. Results: Using 4-mercaptobenzoic acid as the SERS probe molecule, the substrate exhibited good homogeneity, SERS enhancement effect, reproducibility and stability. the SERS signal intensities at the characteristic peaks at 475, 619 cm-1 showed good linear relationships with the logarithms of the concentrations of gentamicin sulphate and neomycin sulphate, respectively （R2 of 0.991 6 and 0.990 7, respectively）. The limits of detections （LODs） were as low as 1×10-9, 1×10-8 mol/L, respectively, and were successfully applied to the rapid, quantitative and high-throughput determination of aminoglycoside antibiotics in pork. Conclusion: The experimental method provides an economical, efficient, time-saving and highly sensitive way for the application of SERS technology for the detection of antibiotic residues in real meat samples.
Publication Date
4-30-2024
First Page
68
Last Page
74,81
DOI
10.13652/j.spjx.1003.5788.2023.80795
Recommended Citation
Haifan, YANG; Li, DING; Miaowen, XU; Kang, SHEN; and Xubo, WANG
(2024)
"Detection of aminoglycoside antibiotic residues in pork by QuEChERs method combined with SERS technique,"
Food and Machinery: Vol. 40:
Iss.
3, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2023.80795
Available at:
https://www.ifoodmm.cn/journal/vol40/iss3/9
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