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Corresponding Author(s)

顾欣（1983—），女，重庆三峡学院副教授，博士。E-mail：shipinzhiliang03@163.com

Abstract

Objective: This study aimed to optimize the preparation process of polysaccharides from lemon peel and screen out the components with high hypoglycemic activity. Methods: Ultrasonic assisted enzymatic extraction of lemon peel polysaccharide, combined with response surface test to explore the best extraction process, through DEAE-52 anion chromatography and Sephadex G-100 gel column chromatography separation and purification to obtain a homogeneous polysaccharide fraction (named as LPs-1a). The effective hypoglycemic components of lemon peel polysaccharides were screened by α-glucosidase and their relative molecular weight, monosaccharide composition and characteristic groups were analyzed. Results: The optimal extraction process of lemon peel polysaccharide was ultrasonic treatment for at 55 ℃ for 44 min with solid-liquid ratio 1∶20 (g/mL) and cellulase addition rate 1.1%. Under the control of these conditions, the yield of lemon peel polysaccharide was 6.83%, which was close to the predicted value. The LPs-1a fraction significantly inhibited the activity of α-glucosidase with （1.22±0.04） mg/mL of IC50(P＜0.05). The relative molecular weight of this component was 195 260, and it was connected by α-glycosidic bonds and β-glycosidic bonds with a pyranose ring. The morphology of the polysaccharide was fibrous, and it was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose Conclusion: Ultrasound-assisted enzymatic method is helpful in screening the highly hypoglycemic active components of lemon peel polysaccharides.

Publication Date

5-21-2024

First Page

187

Last Page

195

DOI

10.13652/j.spjx.1003.5788.2023.81046

References

[1] 农仲文, 于立梅, 曾晓房, 等. 柠檬营养成分及其综合利用研究进展[J]. 农产品加工, 2018（10）: 73-76. NONG Z W, YU L M, ZENG X F, et al. Nutrient composition of lemon and its comprehensive application[J]. Farm Products Processing, 2018（10）: 73-76.
[2] 陈婷, 段宙位. 柠檬皮中多酚的超声辅助提取及其抗氧化性研究[J]. 食品科技, 2023, 48（2）: 246-252. CHEN T, DUAN Z W. Ultrasonic-assisted extraction and antioxidant activity of polyphenols from lemon peel[J]. Food Science and Technology, 2023, 48（2）: 246-252.
[3] PRESENTATO A, SCURRIA A, ALBANESE L, et al. Superior antibacterial activity of integral lemon pectin extracted via hydrodynamic cavitation[J]. Chemistry Open, 2020, 9（5）: 628-630.
[4] SUN Y J, HE Y, WANG F, et al. Low-methoxyl lemon pectin attenuates inflammatory responses and improves intestinal barrier integrity in caerulein-induced experimental acute pancreatitis[J]. Molecular Nutrition & Food Research, 2017, 61（4）: 1600885.
[5] OBOH G, OLASEHINDE T A, ADEMOSUN A O. Inhibition of enzymes linked to type-2 diabetes and hypertension by essential oils from peels of orange and lemon[J]. International Journal of Food Properties, 2017, 20: S586-S594.
[6] 韩晓磊, 吕慧英, 梁珏钦, 等. 春生田头菇粗多糖超声辅助提取及抗氧化性测定[J]. 食品与机械, 2023, 39（9）: 169-176. HAN X L, LU H Y, LIANG J Q, et al. Ultrasound-assisted extraction and antioxidant study of crude polysaccharide from Agrocybe praecox[J]. Food & Machinery, 2023, 39（9）: 169-176.
[7] BIAN Z Y, ZHANG R, ZHANG X, et al. Extraction, structure and bioactivities of polysaccharides from Rehmannia glutinosa: A review[J]. Journal of Ethnopharmacology, 2023, 305: 116132.
[8] CHENG N, WANG H, HAO H F, et al. Research progress on polysaccharide components of Cistanche deserticola as potential pharmaceutical agents[J]. European Journal of Medicinal Chemistry, 2023, 245: 114892.
[9] HASSAN I, GANI A, AHMAD M, et al. Extraction of polysaccharide from Althearosea and its physicochemical, anti-diabetic, anti-hypertensive and antioxidant properties[J]. Scientific Reports, 2022, 12（1）: 17116.
[10] HOSSAIN U, DAS A K, GHOSH S, et al. An overview on the role of bioactive α-glucosidase inhibitors in ameliorating diabetic complications[J]. Food and Chemical Toxicology, 2020, 145: 111738.
[11] 魏鑫悦, 陈克保, 关统伟. 攀枝花黑松露多糖的抗氧化和降血糖活性[J]. 现代食品科技, 2022, 38（3）: 1-7. WEI X Y, CHEN K B, GUAN T W. Antioxidant and hypoglycemic activity of polysaccharide from Panzhihua black truffle[J]. Modern Food Science and Technology, 2022, 38（3）: 1-7.
[12] KIM S Y. Chemical composition and antioxidant activity of crude polysaccharide from citron （Citrus junos Sieb. Ex TANAKA） seed[J]. Preventive Nutrition and Food Science, 2018, 23（4）: 335-340.
[13] CHENR Z, JIN C G, TONG Z G, et al. Optimization extraction, characterization and antioxidant activities of pectic polysaccharide from tangerine peels[J]. Carbohydrate Polymers, 2016, 136: 187-197.
[14] FANG Y, ZHU C Y, QIU D Y, et al. Activation of RAW264.7 macrophages by an acidic polysaccharide derived from Citrus grandis 'Tomentosa'[J]. International Journal of Biological Macromolecules, 2020, 156: 1 323-1 329.
[15] LIU Y L, DONG M, YANG Z Y, et al. Anti-diabetic effect of citrus pectin in diabetic rats and potential mechanism via PI3K/Akt signaling pathway[J]. International Journal of Biological Macromolecules, 2016, 89: 484-488.
[16] 何俊叶, 刘成, 于宠洋, 等. 黄皮不同部位多糖的结构特性及体外降血糖活性[J]. 食品与机械, 2024, 40（3）: 156-164. HE J Y, LIU C, YU C Y, et al. Structural characteristics and hypoglycemic activity of polysaccharides from different parts ofwampee[J]. Food & Machinery, 2024, 40（3）: 156-164.
[17] 高涛. 川明参多糖的结构解析及对消化酶活性的影响[D]. 重庆: 重庆三峡学院, 2021: 15-26. GAO T. Structure analysis and effect of polysaccharide on digestive enzyme activity of Panax chuanmingensis[D]. Chongqing: Chongqing Three Gorges University, 2021: 15-26.
[18] 顾欣, 高涛, 刘梦雅, 等. 梁平柚柚皮多糖的提取、结构解析及抗氧化能力研究[J]. 食品与发酵工业, 2021, 47（7）: 137-145. GU X, GAO T, LIU M Y, et al. Optimization of extraction technology, structure analysis and antioxidant activity of polysaccharides from Liangping pomelo peel[J]. Food and Fermentation Industries, 2021, 47（7）: 137-145.
[19] LIU N, YANG W N, LI X, et al. Comparison of characterization and antioxidant activity of different citrus peel pectins[J]. Food Chemistry, 2022, 386: 132683.
[20] 秦令祥, 周婧琦, 崔胜文, 等. 超声波协同复合酶法提取香菇多糖的工艺优化[J]. 食品研究与开发, 2018, 39（19）: 63-67. QIN L X, ZHOU J Q, CUI S W, et al. Optimization of ultrasonic-assisted compound enzyme extraction technology of Lentinan[J]. Food Research and Development, 2018, 39（19）: 63-67.
[21] 何传波, 魏好程, 熊何健, 等. 枇杷叶多糖酶法提取工艺优化及其离子交换层析纯化[J]. 食品科学, 2016, 37（8）: 45-50. HE C B, WEI H C, XIONG H J, et al. Optimization of enzymatic extraction of polysaccharides from loquat leaves and purification by ion exchange chromatography[J]. Food Science, 2016, 37（8）: 45-50.
[22] 荆瑞勇, 王丽艳, 张跃文. 从脐橙皮中提取可溶性多糖工艺条件研究[J]. 食品科学, 2009, 30（8）: 90-92. JING R Y, WANG L Y, ZHANG Y W. Study on extraction conditions of soluble polysaccharides from navel orange peel[J].Food Science, 2009, 30（8）: 90-92.
[23] 蒋德旗, 黄利敏, 王艳, 等. 响应面优化纤维素酶法提取桂花多糖工艺及其抗氧活性研究[J]. 食品工业科技, 2015, 36（2）: 271-275, 281. JIANG D Q, HUANG L M, WANG Y, et al. Optimization of enzymatic extraction technology of polysaccharide from Osmanthus fragrans using response surface methodology and investigation on its antioxidant activity[J]. Science and Technology of Food Industry, 2015, 36（2）: 271-275, 281.
[24] FU X T, YANG H H, MA C L, et al. Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries[J]. International Journal of Biological Macromolecules, 2020, 163: 414-422.
[25] WANG L, CHEN C, ZHANG B, et al. Structural characterization of a novel acidic polysaccharide from Rosa roxburghii Tratt fruit and its α-glucosidase inhibitory activity[J]. Food & Function, 2018, 9（7）: 3 974-3 985.
[26] PENG Y Q, ZHANG Z H, CHEN W Y, et al. Structural characterization, α-glucosidase inhibitory activity and antioxidant activity of neutral polysaccharide from apricot （Armeniaca Sibirica L. Lam） kernels[J]. International Journal of Biological Macromolecules, 2023, 238: 124109.
[27] 金辰光. 夏枯草、橘皮中多糖分离纯化、结构及抗氧化活性研究[D]. 长春: 长春师范大学, 2016: 27-34. JIN C G. The Isolation, purification, structure and antioxidant activity of polysaccharides from Prunella vulgaris Linn and tangerine peel[D]. Changchun: Changchun Normal University, 2016: 27-34.
[28] 吴韧, 刘爽, 马宽, 等. 豆腐柴果胶多糖理化指标及乳化性质[J/OL]. 食品与发酵工业. （2023-06-02）[2023-10-15]. https://doi.org/10.13995/j.cnki.11-1802/ts.035332. WU R, LIU S, MA K, et al. Physicochemical indexes and emulsification properties of pectin polysaccharide from Premna microphylla Turcz.[J/OL]. Food and Fermentation Industries. （2023-06-02）[2023-10-15].https://doi.org/10.13995/j.cnki.11-1802/ts.035332.
[29] 赵佳莹, 辛玥, 宋萧萧, 等. 鹰嘴豆多糖分离纯化和结构表征[J]. 食品科学, 2023, 44（8）: 40-45. ZHAO J Y, XIN Y, SONG X X, et al. Isolation, purification and structural characterization of polysaccharides from Chickpea[J]. Food Science, 2023, 44（8）: 40-45.
[30] ZHANG H, CHEN J L, LI J H, et al. Extraction and characterization of RG-I enriched pectic polysaccharides from mandarin citrus peel[J]. Food Hydrocolloids, 2018, 79: 579-586.
[31] GONG P, LONG H, GUO Y X, et al. Isolation, structural characterization and hypoglycemic activities in vitro of polysaccharides from Pleurotus eryngii[J]. Molecules, 2022, 27: 7 140.
[32] ZHOU T, JIANG Y M, WEN L R, et al. Characterization of polysaccharide structure in Citrus reticulate 'Chachi' peel during storage and their bioactivity[J]. Carbohydrate Research, 2021, 508: 108398.
[33] 杨玉洁, 刘静宜, 谭艳, 等. 多糖降血糖活性构效关系及作用机制研究进展[J]. 食品科学, 2021, 42（23）: 355-363. YANG Y J, LIU J Y, TAN Y, et al. Progress in understanding the structure-activity relationship and hypoglycemic mechanism of polysaccharides[J]. Food Science, 2021, 42（23）: 355-363.
[34] 刘艳秋, 王亚茹, 毛迪锐, 等. 轮叶党参多糖的分离纯化及结构研究[J]. 食品与机械, 2023, 39（9）: 162-168, 240. LIU Y Q, WANG Y R, MAO D R, et al. Isolation, purification and structure analysis of polysaccharide from lanceasiabell root[J]. Food & Machinery, 2023, 39（9）: 162-168, 240.
[35] 李佳颖, 高誉, 刘红, 等. 绵马贯众多糖超声提取工艺优化及抗氧化活性分析[J]. 基因组学与应用生物学, 2020, 39（1）: 79-87. LI J Y, GAO Y, LIU H, et al. Optimization of ultrasonic assisted extraction of polysaccharides from male fern rhizome and its antioxidant activity[J]. Genomics and Applied Biology, 2020, 39（1）: 79-87.
[36] 刘宁, 任歌, 贺人杰, 等. 枸杞渣多糖的乙酰化修饰及其表征[J]. 陕西科技大学学报, 2021, 39（5）: 39-43. LIU N, REN G, HE R J, et al. Acetylated modification of Lycium barbarum residue polysaccharide and its characterization[J]. Journal of Shaanxi University of Science & Technology, 2021, 39（5）: 39-43.
[37] LI X, HUANG S M, CHEN X, et al. Structural characteristic of a sulfated polysaccharide from Gracilaria Lemaneiformis and its lipid metabolism regulation effect[J]. Food & Function, 2020, 11（12）: 10 876-10 885.
[38] 张倩, 刘宇, 戴晓婧, 等. 黑木耳多糖结构分析及其保护肝细胞氧化损伤活性[J]. 菌物研究, 2021, 19（3）: 170-176. ZHANG Q, LIU Y, DAI X J, et al. Structural analysis of Auricularia heimuer polysaccharide and its activity in protecting liver cells against oxidative damage[J]. Journal of Fungal Research, 2021, 19（3）: 170-176.
[39] FAN M H, SUN X, QIAN Y L, et al. Effects of metal ions in tea polysaccharides on their in vitro antioxidant activity and hypoglycemic activity[J]. International Journal of Biological Macromolecules, 2018, 113: 418-426.

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