Determination of seven nicotinamide compounds in health products

Authors

WU Ziling, Guangzhou Inspection Testing and Certification Group Co., Ltd., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, ChinaFollow
HE Minheng, Guangzhou Inspection Testing and Certification Group Co., Ltd., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
HUANG Jiale, Guangzhou Inspection Testing and Certification Group Co., Ltd., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
CAI Xiayi, Guangzhou Inspection Testing and Certification Group Co., Ltd., Guangzhou, Guangdong 511447, China;National Quality Testing Center for Processed Food 〔Guangdong〕, Guangzhou, Guangdong 511447, China
SUN Wenwen, Shanghai AB Sciex Analytical Instrument Trading Co., Ltd., 〔Guangdong〕, Guangzhou, Guangdong 510623, China

Abstract

Objective： This study aimed to establish an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of 7 nicotinamide compounds in health food. Methods： The samples were dissolved in 10% methanol water, extracted by ultrasonic, and 10 mmol/L ammonium acetate-acetonitrile was used as the mobile phase of gradient elution. The seven nicotinamide compounds were monitored by ESI, positive and negative ion scanning mode, and multiple reaction monitoring (MRM) mode. Results： The 7 nicotinamide compounds showed good linearity in the mass concentration range, and the correlation coefficients were greater than 0.996. The limit of detection (LOD) was 0.075~0.600 mg/kg, the recovery was 84.6%~108.6% with the relative standard deviation of 2.1%~8.7% (n=6). Conclusion： The method has the advantages of simple operation, high speed, high efficiency, high recovery rateand good precision, and can be used for qualitative and quantitative analysis of nicotinamide and other analogues in health care foods.

Publication Date

7-22-2024

First Page

67

Last Page

72,160

DOI

10.13652/j.spjx.1003.5788.2023.80802

Recommended Citation

Ziling, WU; Minheng, HE; Jiale, HUANG; Xiayi, CAI; and Wenwen, SUN (2024) "Determination of seven nicotinamide compounds in health products," Food and Machinery: Vol. 40: Iss. 5, Article 9.
DOI: 10.13652/j.spjx.1003.5788.2023.80802
Available at: https://www.ifoodmm.cn/journal/vol40/iss5/9

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