•  
  •  
 

Corresponding Author(s)

王纪辉(1988—),男,贵州省核桃研究所副研究员,硕士。E-mail:1833247257@qq.com

Abstract

[Objective] This study aimed to investigate the chemical constituents of walnut leaves. [Methods] The volatile components of walnut leaves were analyzed by head-space solid micro-extraction and coupled with GC-MS. The chemical composition of ethanol extract was identified using ultra-high performance liquid chromatography coupled with quadrupole Orbitrap high resolution mass spectrometry (UPLC-Q-Exactive). [Results] 88 kinds of volatile compounds were identified in walnut leaves, mainly terpenoids, of which sesquiterpenoids account for 78.35%. 30 kinds of active components were identified from ethanol extracts, including twenty kinds of polyphenols (twelve kinds of flavonoids), four kinds of phenylpropanoids, three kinds of pentacyclic triterpenoids, two kinds of essential amino acids and 1 tricarboxylic acid compounds in the ethanol extract in walnut leaves. [Conclusion] The combined use of SPME-GC-MS and UPLC-Q Exactive technology can determine and analyze the chemical composition of walnut leaves systematically, accurately and rapidly.

Publication Date

7-22-2024

First Page

164

Last Page

169

DOI

10.13652/j.spjx.1003.5788.2023.60197

References

[1] 马庆国, 乐佳兴, 裴东, 等. 新中国果树科学研究70年——核桃[J]. 果树学报, 2019, 36(10): 1 360-1 368. MA Q G, YUE J X, PEI D, et al. Fruit scientific research in New China in the past 70 years: walnut[J]. Journal of Fruit Science, 2019, 36(10): 1 360-1 368.
[2] 魏欢, 颜小捷, 杨建文, 等. 核桃叶多酚含量的测定和抗氧化能力的研究[J]. 广西植物, 2018, 38(5): 596-601. WEI H, YAN X J, YANG J W, et al. Determination of polyphenols content and antioxidant capacity of walnut leaf[J]. Guihaia, 2018, 38(5): 596-601.
[3] YAO D L, ZHANG C H, LUO J, et al. Chemical constituents from the leaves of Juglans mandshurica[J]. Archives of Pharmacal Research, 2015, 38(4): 480-484.
[4] HOSSEINI S, HUSEINI H F, LARIJANI B, et al. The hypoglycemic effect of Juglans regia leaves aqueous extract in diabetic patients: a first human trial[J]. DARU Journal of Pharmaceutical Sciences, 2014, 22(1): 19-23.
[5] HOSSEINZADEH H, ZAREI H, TAGHIABADI E. Antinociceptive, anti-inflammatory and acute toxicity effects of Juglans regia L. leaves inmice[J]. Iran Red Crescent Med J, 2011, 13(1): 27-33.
[6] PARK G, JANG D S, OH M S. Juglans mandshurica leaf extract protects skin fibroblasts from damage by regulating the oxidative defensesystem[J]. Biochem Biophys Res Commun, 2012, 421(2): 343-348.
[7] 陈超, 胡钰, 孙家祥, 等. 野核桃叶化学成分研究[J]. 中草药, 2011, 42(11): 2 177-2 180. CHEN C, HU Y, SUN J X, et al. Chemical constituents from leaves of Juglans cathayensis[J]. Chinese Traditional and Herbal Drugs, 2011, 42(11): 2 177-2 180.
[8] GIRZU M, CARNAT A, PRIVAT A M, et al. Sedative effect of walnut leaf extract and juglone, an isolated constituent[J]. Pharmaceutical Biology, 1998, 36(4): 280-286.
[9] 刘基, 赵宁, 王小双, 等. 几种云南核桃不同部位总黄酮含量的测定[J]. 山东农业科学, 2017, 49(12): 135-138. LIU J, ZHAO N, WANG X S, et al. Determination of total flavonoid contents in different parts of several species of Yunnan walnuts[J]. Shandong Agricultural Sciences, 2017, 49(12): 135-138.
[10] 刘亚敏, 胥耀平, 高锦明, 等. GC/MS 法分析核桃叶挥发油化学成分[J]. 西北植物学报, 2004, 24(6): 1 100-1 102. LIU Y M, XU Y P, GAO J M, et al. Analysis of volatile components from leaves of Juglans regia by GC/MS[J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(6): 1 100-1 102.
[11] 周晔, 王伟, 王成章, 等. 核桃属(Juglans)植物多酚类物质研究进展[J]. 南京林业大学学报(自然科学版), 2013, 56(5): 146-152. ZHOU Y, WANG W, WANG C Z, et al. Research progress on polyphenols from Juglans plants[J]. Journal of Nanjing Forestry University (Natural Science Edition), 2013, 56(5): 146-152.
[12] 刘晓慧, 张丽霞, 王日为, 等. 顶空固相微萃取—气相色谱—质谱联用法分析黄茶香气成分[J]. 食品科学, 2010, 31(16): 239-243. LIU X H, ZHANG L X, WANG R W, et al. Analysis of volatile components of yellow tea by HS-SPME/GC-MS[J]. Food Science, 2010, 31(16): 239-243.
[13] 李维妮, 郭春锋, 张宇翔, 等. 气相色谱—质谱法分析乳酸菌发酵苹果汁香气成分[J]. 食品科学, 2017, 38(4): 146-154. LI W N, GUO C F, ZHANG Y X, et al. GC-MS Analysis of aroma components of apple juice fermented with lactic acid bacteria[J]. Food Science, 2017, 38(4): 146-154.
[14] 黄秋颜, 李斌, 林晓蓉, 等. 基于高分辨质谱和网络药理学的可可茶多酚降血糖活性研究[J]. 食品与机械, 2023, 39(9): 4-11. HUANG Q Y, LI B, LIN X R, et al. Studying on the hypoglycemic activity of green tea polyphenols from Camellia ptilophylla Chang using high-resolution mass spectrometry and network pharmacology[J]. Food & Machinery, 2023, 39(9): 4-11.
[15] 孙梦佳, 王焕军, 丁富娟, 等. 基于UPLC-Q-Exactive Orbitrap MS和质量亏损过滤技术的三叶青黄酮类化合物定性分析[J]. 质谱学报, 2020, 41(4): 359-367. SUN M J, WANG H J, DING F J, et al. Qualitative analysis of flavonoids in tertastigma hemsleyanum based on UPLC-Quadrupole/exactive orbitrap mass spectrometry and mass defect filter method[J]. Journal of Chinese Mass Spectrometry Society, 2020, 41(4): 359-367.
[16] 孙燕, 冯峰, 黄特辉, 等. 基于UPLC-Q-Exactive技术结合OTCML数据库快速分析沙棘的功效成分[J]. 天然产物研究与开发, 2019, 31(7): 1 192-1 202. SUN Y, FENG F, HUANG T H, et al. Rapid analysis of functional components of Hippophae rhamnoides by UPLC-Q-Exactive and OTCML database[J]. Natural Product Research and Development, 2019, 31(7): 1 192-1 202.
[17] 张巍, 吴亚猛, 陈思瑶, 等. 胡桃醌对宫颈癌SiHa细胞增殖及细胞周期的影响[J]. 吉林大学学报(医学版), 2015, 41(4): 697-700. ZHANG W, WU Y M, CHEN S Y, et al. Effects of Juglone on proliferation and cell cycle of human cervical cancer SiHa cells[J]. Journal of Jilin University (Medicine Edition), 2015, 41(4): 697-700.
[18] 邹翔, 曲中原, 季宇彬. 胡桃醌经由活性氧介导JNK、p38通路诱导SGC-7901细胞凋亡[J]. 中国药学杂志, 2016, 51(7): 544-549. ZOU X, QU Z Y, JI Y B. Juglone induces apoptosis of human gastric cancer SGC-7901 cells through reactive oxygen species me- diated JNK and p38 pathway[J]. Chinese Pharmaceutical Journal, 2016, 51(7): 544-549.
[19] 李晓颍, 武军凯, 王海静. 欧李果实发育期内挥发性成分变化特征[J]. 中国农业科学, 2021, 54(9): 1 964-1 980. LI X Y, WU J K, WANG H J. Characterization of volatiles changes in Chinese dwarf cherry fruit during its development[J]. Scientia Agricultura Sinica, 2021, 54(9): 1 964-1 980.
[20] 江珊珊, 邓青, 范佳. 麦长管蚜对E-β-法尼烯的嗅觉行为反应[J]. 昆虫学报, 2015, 58(7): 776-782. JIANG S S, DENG Q, FAN J. Behavioral responses of Sitobion avenae (Hemiptera: Aphididae) to E-β-farnesene[J]. Scientia Agricultura Sinica, 2015, 58(7): 776-782.
[21] 张书锋, 董庆峰, 胡聪, 等. 石家庄野生荆条花挥发油的化学成分[J]. 中国实验方剂学杂志, 2013, 19(23): 151-153. ZHANG S F, DONG Q F, HU C, et al. Chemical composition of flower volatile oil of wild vitex negundo var heterophylla from Shijiazhuang[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2013, 19(23): 151-153.
[22] 赵杨, 程力, 杜庭, 等. 基于HS-SPME/GC/MS的姜花不同部位挥发性成分分析[J]. 食品与机械, 2020, 36(3): 43-47. ZHAO Y, CHENG L, DU T, et al. Analysis on volatile components in different parts of Hedychium coroarium by HS-SPME/GC/MS[J]. Food & Machinery, 2020, 36(3): 43-47.
[23] 宋诗清, 童彦尊, 冯涛, 等. 金佛手香气物质的多维分析及其特征香气物质的确定[J]. 食品科学, 2017, 38(24): 94-100. SONG S Q, TONG Y Z, FENG T, et al. Multidimensional analysis of odorous compounds in finger citron fruit (Citrus medica L. var. sarcodactylis Swingle) and Identification of key aroma compounds[J]. Food Science, 2017, 38(24): 94-100.
[24] 高夏洁, 高海燕, 赵镭. SPME-GC-MS结合OAV分析不同产区花椒炸花椒油的关键香气物质[J]. 食品科学, 2022, 43(4): 208-214. GAO X J, GAO H Y, ZHAO L. Analysis of the key aroma components of fried Zanthoxylum bungeanum oil from different producing areas based on SPME-GC-MS and OAV[J]. Food Science, 2022, 43(4): 208-214.
[25] 王艳, 周长远, 杜爱玲. 硅胶—硝酸银硅胶柱层析分离纯化β-倍半水芹烯[J]. 食品与机械, 2016, 32(5): 165-167. WANG Y, ZHOU C Y, DU A L. Separation of β-sesquiphellandrene by sillca gel and argentation column chromatography[J]. Food & Machinery, 2016, 32(5): 165-167.
[26] 潘亚琴, 李全清, 张森尧, 等. 山核桃叶与美国山核桃叶中化学成分的初步分析[J]. 中华中医药学刊, 2008, 26(11): 2 517-2 519. PAN Y Q, LI Q Q, ZHANG S Y, et al. Study on chemical components in the leaves of Carya cathayensis and C. illinonsis[J]. Chinese Archives of Traditional Chinese Medicine, 2008, 26(11): 2 517-2 519.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.