•  
  •  
 

Corresponding Author(s)

陈茂龙(1986—),男,长沙理工大学副教授,硕士生导师,博士。E-mail: mlchen@xmu.edu.cn

Abstract

In recent years, with the emergence of resistance to traditional antibiotics and the growing interest in the development of natural antimicrobials, natural antimicrobial peptides have received extensive attention. Among them, plant-derived antimicrobial peptides have the characteristics of low drug resistance, wide antibacterial spectrum and low toxicity, and because of rich cysteine residues, they can form multiple disulfide bonds, so they have high chemical, thermal and enzymatic hydrolysis stability. In this paper, the classification of plant antimicrobial peptides and the methods of extracting and screening antimicrobial peptides from plant sources were reviewed. The potential applications of plant antimicrobial peptides in the field of food were emphasized, and the screening and future applications of these plant antimicrobial peptides in the field of food were prospected.

Publication Date

9-11-2024

First Page

200

Last Page

207,215

DOI

10.13652/j.spjx.1003.5788.2024.80476

References

[1] QIU M Y, ZHENG M, ZHANG J W, et al. Recent advances on emerging biosensing technologies and on-site analytical devices for detection of drug-resistant foodborne pathogens[J]. Trends in Analytical Chemistry, 2023, 167: 117258.
[2] MA B, GUO Y X, FU X, et al. Identification and antimicrobial mechanisms of a novel peptide derived from egg white ovotransferrin hydrolysates[J]. LWT-Food Science and Technology, 2020, 131: 109720.
[3] BAINDARA P, MANDAL S M. Plant-derived antimicrobial peptides: novel preservatives for the food industry[J]. Foods, 2022, 11(16): 2 415.
[4] DEO S, TURTON K L, KAINTH T, et al. Strategies for improving antimicrobial peptide production[J]. Biotechnology Advances, 2022, 59: 107968.
[5] RAMAZI S, MOHAMMADI N, ALLAHVERDI A, et al. A review on antimicrobial peptides databases and the computational tools[J]. Database, 2022, 2 022: 1-17.
[6] YANG S, YUAN Z J, AWEYA J J, et al. Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags[J]. Food Science and Human Wellness, 2022, 11(6): 1 607-1 613.
[7] HE L, ZOU L K, YANG Q R, et al. Antimicrobial activities of nisin, tea polyphenols, and chitosan and their combinations in chilled mutton[J]. Journal of Food Science, 2016, 81(6): M1 466-M1 471.
[8] WANG N, YU X, KONG Q J, et al. Nisin-loaded polydopamine/hydroxyapatite composites: biomimetic synthesis, and in vitro bioactivity and antibacterial activity evaluations[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2020, 602: 125101.
[9] HIRAKI J, ICHIKAWA T, NINOMIYA S I, et al. Use of ADME studies to confirm the safety of ε-polylysine as a preservative in food[J]. Regulatory Toxicology and Pharmacology, 2003, 37(2): 328-340.
[10] KUMARESAN V, KAMARAJ Y, SUBRAMANIYAN S, et al. Understanding the dynamics of human defensin antimicrobial peptides: pathogen resistance and commensal induction[J/OL]. Applied Biochemistry and Biotechnology. (2024-03-13)[2024-05-22]. https://doi.org/10.1007/s12010-024-04893-8.
[11] TAM J, WANG S J, WONG K, et al. Antimicrobial peptides from plants[J]. Pharmaceuticals, 2015, 8(4): 711-757.
[12] OKADA T, YOSHIZUMI H. A lethal toxic substance for brewing yeast in wheat and barley: part II isolation and some properties of toxic principle[J]. Agricultural and Biological Chemistry, 1970, 34(7): 1 089-1 094.
[13] HEYMICH M L, FRIEDLEIN U, TROLLMANN M, et al. Generation of antimicrobial peptides Leg1 and Leg2 from chickpea storage protein, active against food spoilage bacteria and foodborne pathogens[J]. Food Chemistry, 2021, 347: 128917.
[14] VASILCHENKO A S, SMIRNOV A N, ZAVRIEV S K, et al. Novel thionins from black seed (Nigella sativa L.) demonstrate antimicrobial activity[J]. International Journal of Peptide Research and Therapeutics, 2016, 23(2): 171-180.
[15] AZMI S, HUSSAIN M K. Analysis of structures, functions, and transgenicity of phytopeptides defensin and thionin: a review[J]. Beni-Suef University Journal of Basic and Applied Sciences, 2021, 10(1): 1-11.
[16] SOHRABI S M, SHAHMOHAMMADI M, MOHAMMADI M, et al. Identification and functional characterization a cysteine-rich peptide from the garlic (Allium sativum L.)[J]. South African Journal of Botany, 2024, 166: 690-697.
[17] SLAVOKHOTOVA A A, SHELENKOV A A, ANDREEV Y A, et al. Hevein-like antimicrobial peptides of plants[J]. Biochemistry (Moscow), 2018, 82(13): 1 659-1 674.
[18] GAMES P D, DASILVA E Q G, BARBOSA M D O, et al. Computer aided identification of a Hevein-like antimicrobial peptide of bell pepper leaves for biotechnological use[J]. BMC Genomics, 2016, 17: 999.
[19] SHAO F, HU Z, XIONG Y M, et al. A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning[J]. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1999, 1 430(2): 262-268.
[20] SLAVOKHOTOVA A A, ROGOZHIN E A. Defense peptides from the α-hairpinin family are components of plant innate immunity[J]. Frontiers in Plant Science, 2020, 11: 465.
[21] BARASHKOVA A S, RYAZANTSEV D Y, ROGOZHIN E A. Rational design of plant hairpin-like peptide EcAMP1: structural-functional correlations to reveal antibacterial and antifungal activity[J]. Molecules, 2022, 27(11): 3 554.
[22] CUI X D, DU J J, LI J, et al. Inhibitory site of α-hairpinin peptide from tartary buckwheat has no effect on its antimicrobial activities[J]. Acta Biochimica et Biophysica Sinica, 2018, 50(4): 408-416.
[23] AMADOR V C, SANTOS-SILVA C A D, VILELA L M B, et al. Lipid transfer proteins (LTPs)-structure, diversity and roles beyond antimicrobial activity[J]. Antibiotics, 2021, 10(11): 1 281.
[24] BARASHKOVA A S, SMIRNOV A N, ZORINA E S, et al. Diversity of cationic antimicrobial peptides in black cumin (Nigella sativa L.) seeds[J]. International Journal of Molecular Sciences, 2023, 24(9): 8 066.
[25] SU T, HAN M, CAO D, et al. Molecular and biological properties of snakins: the foremost cysteine-rich plant host defense peptides[J]. Journal of Fungi, 2020, 6(4): 220.
[26] SHANG C J, YE T, ZHOU Q, et al. Genome-wide identification and bioinformatics analyses of host defense peptides snakin/GASA in mangrove plants[J]. Genes, 2023, 14(4): 923.
[27] 郑兰兰, 陆强, 刘丹丹, 等. 植物抗菌肽的抗肿瘤活性及其临床治疗展望[J]. 国外医药抗生素分册, 2017, 38(6): 279-283. ZHENG L L, LU Q, LIU D D, et al. Research progress of plant antimicrobial peptides with antitumor activity[J]. World Notes on Antibiotics, 2017, 38(6): 279-283.
[28] CONZELMANN C, MURATSPAHIC E, TOMAEVIC N, et al. In vitro inhibition of HIV-1 by cyclotide-enriched extracts of viola tricolor[J]. Frontiers in Pharmacology, 2022, 13: 888961.
[29] 田野, 王贵锋, 张向前. 植物抗菌肽的研究进展及其应用[J]. 现代食品科技, 2017, 33(11): 285-291. TIAN Y, WANG G F, ZHANG X Q. Research progress and application of plant antimicrobial peptides[J]. Modern Food Science and Technology, 2017, 33(11): 285-291.
[30] ZOU F L, TAN C M, SHINALI T S, et al. Plant antimicrobial peptides: a comprehensive review of their classification, production, mode of action, functions, applications, and challenges[J]. Food & Function, 2023, 14(12): 5 492-5 515.
[31] BARASHKOVA A S, ROGOZHIN E A. Isolation of antimicrobial peptides from different plant sources: does a general extraction method exist?[J]. Plant Methods, 2020, 16(1): 143.
[32] SONG J J, PENG S D, YANG J, et al. Isolation and identification of novel antibacterial peptides produced by Lactobacillus fermentum SHY10 in Chinese pickles[J]. Food Chemistry, 2021, 348: 129097.
[33] 张琦, 王凤扬, 鄢梦, 等. 山苍子蛋白抗菌肽的抑菌特性及稳定性研究[J]. 中国测试, 2023, 49(8): 81-86. ZHANG Q, WANG F Y, YAN M, et al. Study on antibacterial properties and stability of antibacterial peptides from Litsea cubeba protein[J]. China Measurement & Test, 2023, 49(8): 81-86.
[34] 江晨, 齐宏涛, 于丽娜, 等. 响应面优化花生蛋白抗菌肽制备工艺[J]. 山东农业科学, 2021, 53(11): 111-119. JIANG C, QI H T, YU L N, et al. Optimization of peanut antibacterial peptide preparation by response surface methodology[J]. Shandong Agricultural Sciences, 2021, 53(11): 111-119.
[35] ECHAVE J, FRAGA-CORRAL M, GARCIA-PEREZ P, et al. Seaweed protein hydrolysates and bioactive peptides: extraction, purification, and applications[J]. Marine Drugs, 2021, 19(9): 500.
[36] 费丛璇, 付美玲, 张迪, 等. 果胶的提取、生理功能及应用研究进展[J]. 食品与机械, 2024, 40(3): 233-240. FEI C X, FU M L, ZHANG D, et al. Research progress on extraction, physiological function and application of pectin[J]. Food & Machinery, 2024, 40(3): 233-240.
[37] AGUILAR-TOAL J E, DEERING A J, LICEAGA A M. New insights into the antimicrobial properties of hydrolysates and peptide fractions derived from chia seed (Salvia hispanica L.)[J]. Probiotics and Antimicrobial Proteins, 2020, 12(4): 1 571-1 581.
[38] LIU D W, LIU M, MENG D H, et al. Harsh sensitivity and mechanism exploration of an antibacterial peptide extracted from walnut oil residue derived from agro-industrial waste[J]. Journal of Agricultural and Food Chemistry, 2022, 70(24): 7 460-7 470.
[39] UM J, MANGUY J, ANES J, et al. Enriching antimicrobial peptides from milk hydrolysates using pectin/alginate food-gels[J]. Food Chemistry, 2021, 352: 129220.
[40] NIU C, SONG X Y, ZHANG Y X, et al. A rapid one-step process for the isolation of antibacterial peptides by silica-decorated Fe3O4 nanoparticles[J]. LWT-Food Science and Technology, 2022, 155: 112858.
[41] SONG W G, KONG X Z, HUA Y F, et al. Identification of antibacterial peptides generated from enzymatic hydrolysis of cottonseed proteins[J]. LWT-Food Science and Technology, 2020, 125: 109199.
[42] TANG S S, PRODHAN Z H, BISWAS S K, et al. Antimicrobial peptides from different plant sources: isolation, characterisation, and purification[J]. Phytochemistry, 2018, 154: 94-105.
[43] SCHMIDT M, ARENDT E K, THERY T L C. Isolation and characterisation of the antifungal activity of the cowpea defensin Cp-thionin II[J]. Food Microbiology, 2019, 82: 504-514.
[44] WANG X F, HE L, HUANG Z Y, et al. Isolation, identification and characterization of a novel antimicrobial peptide from Moringa oleifera seeds based on affinity adsorption[J]. Food Chemistry, 2023, 398: 133923.
[45] DANG T T, TRAN T T T, TRAN G H, et al. Cyclotides derived from Viola dalatensis gagnep: a novel approach for enrichment and evaluation of antimicrobial activity[J]. Toxicon, 2024, 239: 107606.
[46] XU K J, ZHAO X Y, TAN Y M, et al. A systematical review on antimicrobial peptides and their food applications[J]. Biomaterials Advances, 2023, 155: 213684.
[47] HUANG Y N, GAO L G, LIN M, et al. Recombinant expression of antimicrobial peptides in Pichia pastoris: a strategy to inhibit the Penicillium expansum in pears[J]. Postharvest Biology and Technology, 2021, 171: 111298.
[48] BARASHKOVA A S, RYAZANTSEV D Y, ZHURAVLEVA A S, et al. Recombinant fusion protein containing plant nigellothionin regulates the growth of food-spoiling fungus (Aspergillus niger)[J]. Foods, 2023, 12(16): 3 002.
[49] 薛凤, 封硕, 李菁. 人工智能方法在抗菌肽筛选领域的应用及展望[J]. 中国医科大学学报, 2023, 54(3): 314-322. XUE F, FENG S, LI J. Application and prospect of artificial intelligence in antimicrobial peptides screening[J]. Journal of China Pharmaceutical University, 2023, 54(3): 314-322.
[50] XU J Q, XU X, JIANG Y H, et al. Waste to resource: mining antimicrobial peptides in sludge from metagenomes using machine learning[J]. Environment International, 2024, 186: 108574.
[51] GAN B H, GAYNORD J, ROWE S M, et al. The multifaceted nature of antimicrobial peptides: current synthetic chemistry approaches and future directions[J]. Chemical Society Reviews, 2021, 50(13): 7 820-7 880.
[52] PANDI A, ADAM D, ZARE A, et al. Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides[J]. Nature Communications, 2023, 14(1): 7 197.
[53] ZHANG Y, LIU L H, XU B, et al. Screening antimicrobial peptides and probiotics using multiple deep learning and directed evolution strategies[J/OL]. Acta Pharmaceutica Sinica B. (2024-05-06)[2024-05-22]. https://doi.org/10.1016/j.apsb.2024.05.003.
[54] 杨悦, 李燕, 王小方, 等. 抗菌肽及其在食物储藏与保鲜中的应用[J]. 食品与生物技术学报, 2021, 40(4): 9-16. YANG Y, LI Y, WANG X F, et al. Antimicrobial peptides and their applications in food storage and preservation[J]. Journal of Food Science and Biotechnology, 2021, 40(4): 9-16.
[55] HUAN Y C, KONG Q, MOU H J, et al. Antimicrobial peptides: classification, design, application and research progress in multiple fields[J]. Frontiers in Microbiology, 2020, 11: 582779.
[56] SUN A D, HUANG Z Y, HE L, et al. Metabolomic analyses reveal the antibacterial properties of a novel antimicrobial peptide MOp3 from Moringa oleifera seeds against Staphylococcus aureus and its application in the infecting pasteurized milk[J]. Food Control, 2023, 150: 109779.
[57] 王丽芳, 叶良, 谢忠稳, 等. 茶叶抗菌肽粗提物的抑菌活性及其对冷却肉保鲜的影响[J]. 浙江农业学报, 2022, 34(10): 2 268-2 276. WANG L F, YE N, XIE Z W, et al. Antibacterial activity of tea antimicrobial peptide extraction and its effect on preservation of chilled meat[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2 268-2 276.
[58] 王宏亮, 李昂, 那杰. 抗菌肽在果蔬保鲜中的应用[J]. 江苏农业科学, 2014, 42(3): 238-240. WANG H L, LI A, NA J. Application of antimicrobial peptides in fruit and vegetable preservation[J]. Jiangsu Agricultural Sciences, 2014, 42(3): 238-240.
[59] RIVERO-PINO F, LEON M J, MILLAN-LINARES M C, et al. Antimicrobial plant-derived peptides obtained by enzymatic hydrolysis and fermentation as components to improve current food systems[J]. Trends in Food Science & Technology, 2023, 135: 32-42.
[60] LIU Y W, SAMEEN D E, AHMED S, et al. Antimicrobial peptides and their application in food packaging[J]. Trends in Food Science & Technology, 2021, 112: 471-483.
[61] JAMROZ E, KULAWIK P, TKACZEWSKA J, et al. The effects of active double-layered furcellaran/gelatin hydrolysate film system with Ala-Tyr peptide on fresh Atlantic mackerel stored at -18 ℃[J]. Food Chemistry, 2021, 338: 127867.
[62] 唐田园. 苦荞抗菌肽/多糖复合膜的制备及其在牛肉糜保鲜中的应用[D]. 昆明: 昆明理工大学, 2019: 74. TANG T Y. Preparation of bitter buckwheat antimicrobial peptide/polysaccharide composite membrane and its application in fresh-keeping of ground beef[D]. Kunming: Kunming University of Science and Technology, 2019: 74.
[63] OLIVEIRA J T A, SOUZA P F N, VASCONCELOS I M, et al. Mo-CBP3-PepI, Mo-CBP3-PepII, and Mo-CBP3-PepIII are synthetic antimicrobial peptides active against human pathogens by stimulating ROS generation and increasing plasma membrane permeability[J]. Biochimie, 2019, 157: 10-21.
[64] LIMA P G, FREITAS C D T, OLIVEIRA J T A, et al. Synthetic antimicrobial peptides control Penicillium digitatum infection in orange fruits[J]. Food Research International, 2021, 147: 110582.
[65] SHWAIKI L N, SAHIN A W, ARENDT E K. Study on the inhibitory activity of a synthetic defensin derived from barley endosperm against common food spoilage yeast[J]. Molecules, 2020, 26(1): 165.
[66] SHWAIKI L N, ARENDT E K, LYNCH K M. Study on the characterisation and application of synthetic peptide snakin-1 derived from potato tubers-action against food spoilage yeast[J]. Food Control, 2020, 118: 107362.
[67] YI L H, QI T, MA J H, et al. Genome and metabolites analysis reveal insights into control of foodborne pathogens in fresh-cut fruits by Lactobacillus pentosus MS031 isolated from Chinese Sichuan Paocai[J]. Postharvest Biology and Technology, 2020, 164: 111150.
[68] SHWAIKI L N, ARENDT E K, LYNCH K M. Anti-yeast activity and characterisation of synthetic radish peptides Rs-AFP1 and Rs-AFP2 against food spoilage yeast[J]. Food Control, 2020, 113: 107178.
[69] LEON MADRAZO A, SEGURA CAMPOS M R. In silico prediction of peptide variants from chia (S. hispanica L.) with antimicrobial, antibiofilm, and antioxidant potential[J]. Computational Biology and Chemistry, 2022, 98: 107695.
[70] JIANG J, HOU X, XU K, et al. Bacteria-targeted magnolol-loaded multifunctional nanocomplexes for antibacterial and anti-inflammatory treatment[J]. Biomedical Materials, 2024, 19(2): 025029.
[71] PAVLICEVIC M, MARMIROLI N, MAESTRI E. Immunomodulatory peptides: a promising source for novel functional food production and drug discovery[J]. Peptides, 2022, 148: 170696.
[72] KAMAL I, ASHFAQ U A, HAYAT S, et al. Prospects of antimicrobial peptides as an alternative to chemical preservatives for food safety[J]. Biotechnology Letters, 2022, 45(2): 137-162.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.