Extraction of flavonoids from Goji (Lycium barbarum L.) leaves by ultrasonic-assisted two-phase aqueous method and the inhibitory activity of the extract on pancreatic lipase

Authors

JIA Xiaofeng, School of Food Science and Engineering , Ningxia University , Yinchuan , Ningxia 750021 , China;Ningxia University Institute of Goji Berry Modern Industry , Yinchuan , Ningxia 750021 , China
GAO Die, School of Food Science and Engineering , Ningxia University , Yinchuan , Ningxia 750021 , China;Ningxia University Institute of Goji Berry Modern Industry , Yinchuan , Ningxia 750021 , China
LIU Jinwang, School of Food Science and Engineering , Ningxia University , Yinchuan , Ningxia 750021 , China;Ningxia University Institute of Goji Berry Modern Industry , Yinchuan , Ningxia 750021 , China
XU Yuying, School of Food Science and Engineering , Ningxia University , Yinchuan , Ningxia 750021 , China;Ningxia University Institute of Goji Berry Modern Industry , Yinchuan , Ningxia 750021 , China
FAN Yanli, School of Food Science and Engineering , Ningxia University , Yinchuan , Ningxia 750021 , China;Ningxia University Institute of Goji Berry Modern Industry , Yinchuan , Ningxia 750021 , ChinaFollow

Corresponding Author(s)

范艳丽（1980—），女，宁夏大学教授，博士。E-mail：fanyanli_fyl@163.com

Abstract

［Objective ］ This study aimed to achieve high yield and green extraction of flavonoids from Goji (Lycium barbarum L.) leaves using ultrasonic assisted aqueous two -phase method,and to evaluate the activity of extract.［Methods ］ Single factor and PB (Plackett -Burman ) experiments were conducted to investigate the influence of various factors on the extraction efficiency of flavonoids.Three parameters were chosen to optimize the extraction conditions using a Central Composite Design (CCD ) of the response surface experiment:the amount of polyethylene glycol (PEG 400),the amount of ammonium sulfate,and the solid -liquid ratio.The extract's activity was assessed using an activity inhibition test of pancreatic lipase.［Results］ The optimal process conditions for ultrasonic -assisted aqueous two -phase extraction of flavonoids from Goji berry leaves were:28% polyethylene glycol 400,16% ammonium sulfate,ultrasonic power of 250 W,ultrasonic treatment time of 40 minutes,ultrasonic treatment temperature of 40 ℃,and solid -solid ratio of 1∶30 (g/g).Under these conditions,the total flavonoid content of the extract was (486.30±3.29) mg/g,which had a significant inhibitory effect on pancreatic lipase activity,and the half inhibition concentration (IC50) was 1.06 mg/mL.［Conclusion ］ Under the optimal extraction conditions,the flavonoids extracted from L.barbarum leaves by ultrasonic -assisted aqueous phase method had high yield and low solvent residue,and the flavonoids from L.barbarum leaves obtained by this method had a good inhibitory effect on pancreatic lipase.

Publication Date

2-18-2025

First Page

144

Last Page

151

DOI

10.13652/j.spjx.1003.5788.2023.81047

Recommended Citation

Xiaofeng, JIA; Die, GAO; Jinwang, LIU; Yuying, XU; and Yanli, FAN (2025) "Extraction of flavonoids from Goji (Lycium barbarum L.) leaves by ultrasonic-assisted two-phase aqueous method and the inhibitory activity of the extract on pancreatic lipase," Food and Machinery: Vol. 40: Iss. 8, Article 20.
DOI: 10.13652/j.spjx.1003.5788.2023.81047
Available at: https://www.ifoodmm.cn/journal/vol40/iss8/20

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