Effects of differential protein removal on the properties of early indica rice flour

Authors

CAO Yijun, National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi , Jiangsu 214122 , China;School of Food Science and Technology , Jiangnan University ,Wuxi , Jiangsu 214122 , China
FENG Wei, National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi , Jiangsu 214122 , China;School of Food Science and Technology , Jiangnan University ,Wuxi , Jiangsu 214122 , China
WANG Ren, National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi , Jiangsu 214122 , China;School of Food Science and Technology , Jiangnan University ,Wuxi , Jiangsu 214122 , China
ZHANG Hao, National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi , Jiangsu 214122 , China;School of Food Science and Technology , Jiangnan University ,Wuxi , Jiangsu 214122 , China;College of Food Science and Technology , Nanjing Agricultural University , Nanjing , Jiangsu 210095 , China
WANG Tao, National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi , Jiangsu 214122 , China;School of Food Science and Technology , Jiangnan University ,Wuxi , Jiangsu 214122 , ChinaFollow

Corresponding Author(s)

王涛（1989—），男，江南大学副研究员，博士。E-mail：twang3813@gmail.com

Abstract

［Objective ］ To investigate the mechanism of the effect of deproteinisation on the physicochemical and functional properties of rice flour.［Methods ］ Enzymatic and alkaline methods were used to remove protein from early rice flour,and the damaged starch content,size distribution,morphology,solubility,swelling power,pasting characteristics and textural characteristics of the deproteinized rice flour were analyzed.［Results］ Sodium dodecyl sulfate -polyacrylamide gel electrophoresis (SDS -PAGE ) and confocal laser scanning microscope (CLSM ) showed that the alkaline method equally removed the protein subunit where as the enzymatic method showed a better homogeneity of protein removal from the surface of starch.The size distribution of the control showed a majorpeak at ca.70 μm and it shifted to 6μmafter protein removal.With the reduction of the protein content,and the damaged starch content of the deproteinized rice flour decreased while its elasticity and hardness increased.The deproteinized rice flour with 3.0% of protein (RFA@ 3.0),which was treated by alkaline method,showed a most uniform particle distribution,and a minimum D90 of 23.58 μm was observed.When compared to the control,its damaged starch content decreased from 3.32% to 1.48% while the elasticity and hardness increased by 54.55%,52.60%,respectively.The deproteinized rice flour had lower peak viscosity,breakdown viscosity and final viscosity than the control,and the RFA@ 3.0 had minimum breakdown and setback viscosities,which indicated that it exhibited best paste stability and was difficult to retrograde.［Conclusion ］ The protein removal by alkaline and enzymatic methods can improve the properties of early indica rice flour.The enzymatic method has mild reaction conditions and the result and products has better protein and size distribution and are more difficult to retrograde than the rice flour that treated by alkaline method.

Publication Date

2-18-2025

First Page

16

Last Page

22,39

DOI

10.13652/j.spjx.1003.5788.2024.80232

Recommended Citation

Yijun, CAO; Wei, FENG; Ren, WANG; Hao, ZHANG; and Tao, WANG (2025) "Effects of differential protein removal on the properties of early indica rice flour," Food and Machinery: Vol. 40: Iss. 8, Article 3.
DOI: 10.13652/j.spjx.1003.5788.2024.80232
Available at: https://www.ifoodmm.cn/journal/vol40/iss8/3

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