Abstract
[Objective ] Addressing the heavy metal lead pollution in oysters using near -infrared spectroscopy technology.[Methods ] This study proposed the use of near -infrared reflectance spectroscopy combined with pattern recognition for detecting Pb contamination.Initially,spectral data of healthy mussels and Pb -contaminated mussels in the range of 950~1 700 nm were collected.The wavelength selection algorithm of variable importance analysis based on the random variable combination (VIAVC ) was utilized to reduce the dimensionality,and selected the optimal subset of wavelengths.Considering the detection of healthy mussels and Pb -contaminated mussels as an imbalanced classification problem,the gravitational fixed radius nearest neighbor (GFRNN ) method based on universal gravity was explored for identifying Pb contamination in mussels.[Results] The experimental results demonstrated that the proposed VIAVC -GFRNN method outperformed traditional algorithms such as K -nearest neighbor,fixed radius nearest neighbor,and support vector machine algorithms in detecting Pb contamination,while remaining unaffected by the imbalance ratio.The area under the receiver operation curve value of the VIAVC -GFRNN model reached 0.988 6,with a detection accuracy and geometric mean of 99.17%.[Conclusion ] Near -infrared spectroscopy combined with pattern recognition methods has great potential for detecting Pd pollution in mussels.
Publication Date
2-18-2025
First Page
49
Last Page
57
DOI
10.13652/j.spjx.1003.5788.2024.80001
Recommended Citation
Wei, JIANG; Zhongyan, LIU; Yao, LIU; Jianfang, XIONG; and Shaogeng, ZENG
(2025)
"Identification of heavy metal Pb pollution in Perna viridis based on near-infrared spectroscopy,"
Food and Machinery: Vol. 40:
Iss.
8, Article 7.
DOI: 10.13652/j.spjx.1003.5788.2024.80001
Available at:
https://www.ifoodmm.cn/journal/vol40/iss8/7
References
[1] 张舒玄,卢海燕,李优琴,等.农产品中重金属的检测方法研究进展 [J].理化检验 (化学分册 ),2019,55(8):976-983.ZHANG S X,LU H Y,LI Y Q,et al.Recent advances of researches on detection methods of heavy metals in agricultural products [J].Physical Testing and Chemical Analysis Part B(Chemical Analysis ),2019,55(8):976-983.
[2] 李万杰,马春,张新欣,等.微波消解—石墨炉原子吸收法检测海产品中痕量重金属 [J].大连工业大学学报,2015,34(2):111-113.LI W J,MA C,ZHANG X X,et al.Determination of trace heavy metal in marine products by microware digestion -graphite furnace atomic absorption spectrometry [J].Journal of Dalian Polytechnic University,2015,34(2):111-113.
[3] YAP C K,ISMAIL A,TAN S G,et al.Assessment of different soft tissues of the green -lipped mussel Perna viridis (Linnaeus ) as biomonitoring agents of Pb:field and laboratory studies [J].Water Air Soil Pollut,2004,153(1):253-268.
[4] 孙玲玲,宋金明,刘瑶,等.四极杆碰撞反应池 ICP-MS同时测定贻贝中的 Mo等12种重金属 [J].海洋环境科学,2020,39(3):453-459.SUN L L,SONG J M,LIU Y,et al.Simultaneous determination of molybdenum and other heavy metals in Mytilus edulis by inductively coupled plasma mass spectrometry with quadrupole collision cell technology [J].Marine Environmental Science,2020,39(3):453-459.
[5] 张欣欣,李尚科,李跑,等.近红外漫反射光对水果的穿透能力研究 [J].中国食品学报,2022,22(1):298-305.ZHANG X X,LI S K,LI P,et al.Studies on the penetration ability of near infrared diffuse light on fruits [J].Journal of Chinese Institute of Food Science and Technology,2022,22(1):298-305.
[6] 王海华,李长缨,李民赞.基于近红外反射光谱的洋葱可溶性固体物检测 [J].光谱学与光谱分析,2013,33(9):2 403-2 406.WANG H H,LI C Y,LI M Z.Detection of onion soluble solids content based on the near -infrared reflectance spectra [J].Spectroscopy and Spectral Analysis,2013,33(9):2 403-2 406.
[7] 黄明月,吴海云,靳皓,等.基于近红外透射—漫反射光谱掺杂牛奶判别 [J].光谱学与光谱分析,2020,40(S1):85-86.HUANG M Y,WU H Y,JIN H,et al.Discrimination of adulterated milk based on near infrared transmission and diffuse reflectance spectroscopy [J].Spectroscopy and Spectral Analysis,2020,40(S1):85-86.
[8] 吕程序,姜训鹏,张银桥,等.基于变量选择的小麦粗蛋白含量近红外光谱检测 [J].农业机械学报,2016,47(S1):340-346.LU C X,JIANG X P,ZHANG Y Q,et al.Variable selection based near infrared spectroscopic quantitative analysis on wheat crude protein content [J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(S1):340-346.
[9] 姜微,刘瑶,刘忠艳,等.间隔影响分析波长选择算法在近红外光谱鉴别贝类毒素中的应用 [J].食品与发酵工业,2023,49(2):271-279.JIANG W,LIU Y,LIU Z Y,et al.Application of margin influence analysis wavelength selection algorithm in the identification of shellfish toxins by near infrared spectroscopy[J].Food and Fermentation Industries,2023,49(2):271-279.
[10] 唐鸣,田潇瑜,王旭,等.基于近红外特征波段的注水肉识别模型研究 [J].农业机械学报,2018,49(S1):440-446.TANG M,TIAN X Y,WANG X,et al.Recognition model of water-injected meat based on characteristic spectrum extraction of infrared spectroscopy [J].Transactions of the Chinese society for Agricultural Machinery,2018,49(S1):440-446.
[11] GARCÍA -MARTÍN J F,BADARÓ A T,BARBIN D F,et al.Identification of copper in stems and roots of Jatropha curcas L.by hyperspectral imaging [J].Processes,2020,8(7):822-831.
[12] CHEN Y N,SUN D W,CHENG J H,et al.Recent advances for rapid identification of chemical information of muscle foods by hyperspectral imaging analysis [J].Food Eng Rev,2016,8(3):336-350.|[13] LIU Y,XU L L,ZENG S G,et al.Rapid detection of mussels contaminated by heavy metals using near -infrared refectance spectroscopy and a constrained diference extreme learning machine [J].Spectrochimica Acta Part A,2022,269:120776.
[14] 曾绍庚,刘瑶,刘忠艳.基于近红外光谱技术和 LSPTSVM 模型 的 镉 污 染 贻 贝 检 测 研 究 [J].环 境 工 程,2024,42(1):235-242.ZENG S G,LIU Y,LIU Z Y.Detection of mussels contaminated with cadmium based on near -infrared spectroscopy and lsptsvm [J].Environmental Engineering,2024,42(1):235-242.
[15] ZHU Y J,WANG Z,GAO D Q.Gravitational fixed radius nearest neighbor for imbalanced problem [J].Knowledge -Based Systems,2015,90:224-238.
[16] 程波,彭嘉琪,王新月,等.中国水产品质量安全标准体系现状研究 [J].中国渔业质量与标准,2023,13(5):53-66.CHENG B,PENG J Q,WANG X Y,et al.Research on the current situation of China ’s aquatic product quality and safety standard system [J].Chinese Fishery Quality and Standards,2023,13(5):53-66.
[17] 葛奇伟.养殖贝类重金属特征污染物的筛选及其风险评价[D].宁波:宁波大学,2013:19-23.GE Q W.Studies on characteristic selection of heavy metal pollutants for cultured molluscs and their risk assessment [D].Ningbo:Ningbo University,2013:19-23.
[18] 刘莉,陶红燕,方静,等.基于近红外高光谱的梨叶片炭疽病与黑斑病识别 [J].农业机械学报,2022,53(2):221-230.LIU L,TAO H Y,FANG J,et al.Identifying anthracnose and black spot of pear leaves on near -infrared hyperspectroscopy[J].Transactions of the Chinese Society for Agricultural Machinery,2022,53(2):221-230.
[19] 孙俊,张跃春,毛罕平,等.基于计算机视觉的土壤镉胁迫生菜 叶 片 污 染 响 应 分 析 [J].农 业 机 械 学 报,2018,49(3):166-172.SUN J,ZHANG Y C,MAO H P,et al.Responses analysis of lettuce leaf pollution in cadmium stress based on computer vision [J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(3):166-172.
[20] YUN Y H,FU L,DENG B C,et al.Erratum to:Informative metabolites identification by variable importance analysis based on random variable combination [J].Metabolomics,2016,12(2):1-13.
[21] HOLM S.A simple sequentially rejective multiple test procedure [J].Scandinavian Journal of Statistics,1979,6(2):65-70.
[22] WESTERHUIS J A,HOEFSLOOT H C,SMIT S,et al.Assessment of PLSDA cross validation [J].Metabolomics,2008,4(1):81-89.
[23] 周鹏,伊静,朱振方,等.面向不平衡分类的固定半径最近邻逐步竞争算法 (FRNNPC ) [J].山东大学学报 (理学版 ),2019,54(3):102-109.ZHOU P,YIN J,ZHU Z F,et al.Fixed-radius nearest neighbor progressive competition algorithm for imbalanced classification [J].Journal of Shandong University (Natural Science ),2019,54(3):102-109.
[24] RODRIGUEZ A,LAIO A.Clustering by fast search and find of density peaks [J].Science,2014,344(6 191):1 492-1 496.
[25] 孙通,吴宜青,李晓珍,等.基于近红外光谱和子窗口重排分析的山茶油掺假检测 [J].光学学报,2015,35(6):1-8.SUN T,WU Y Q,LI X Z,et al.Discrimination of camellia oil adulteration by nir spectra and subwindow permutation analysis [J].Acta Optica Sinica,2015,35(6):1-8.
[26] 郑剑,周竹,仲山民,等.基于近红外光谱与随机青蛙算法的褐变板栗识别 [J].浙江农林大学学报,2016,33(2):322-329.ZHENG J,ZHOU Z,ZHONG S M,et al.Chestnut browning detected with near -infrared spectroscopy and a random -frog algorithm [J].Journal of Zhejiang A & F University,2016,33(2):322-329.
[27] COVER T M,HART P E.Nearest neighbor pattern classification [J].IEEE Transactions on Information Theory,1967,13(1):21-27.
[28] COEN T,SAEYS W,RAMON H,et al.Optimizing the tuning parameters of least square support vector machines regression of NIR spectra [J].Journal of Chemometrics,2006,20:184-192.