Determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry

Authors

LIU Xiaofang, Hunan Provincial Institute of Product and Goods Quality Inspection , Changsha , Hunan 410007 , China ;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning , Changsha , Hunan 410007 , ChinaFollow
LI Zheng, Hunan Provincial Institute of Product and Goods Quality Inspection , Changsha , Hunan 410007 , China ;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning , Changsha , Hunan 410007 , China
HE Hao, Hunan Provincial Institute of Product and Goods Quality Inspection , Changsha , Hunan 410007 , China ;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning , Changsha , Hunan 410007 , China
JIANG Junjie, Hunan Provincial Institute of Product and Goods Quality Inspection , Changsha , Hunan 410007 , China ;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning , Changsha , Hunan 410007 , China
CHEN Yuanyuan, Hunan Provincial Institute of Product and Goods Quality Inspection , Changsha , Hunan 410007 , China ;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning , Changsha , Hunan 410007 , China

Corresponding Author(s)

刘小芳（1991—），女，湖南省产商品质量检验研究院工程师，硕士。E-mail：740954546@qq.com

Abstract

［［Objective ］］ To establish a method for simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal -derived food by ultra -high performance liquid chromatography -tandem mass spectrometry.［［Methods ］］ The samples were extracted by oscillating with acetonitrile solution of 1% acetic acid,defatted with n -hexane,purified by matrix dispersible solid phase extractant (PSA,anhydrous magnesium sulfate ),separated by Waters BEH C18 column under a gradient elution program using 0.1% formic acid aqueous solution and acetonitrile as the mobile phases.The anion multiple reaction monitoring model (MRM ) of atmospheric pressure chemical ionization source (APCI ) was used for the determination,and the matrix matching external standard method was used for quantitative determination.［［Results ］］ The linear relationships of chlorothalonil and 4-hydroxychlorothalonil were good in the range of 5~500 μg/L,and the limits of quantitation were 0.005 mg/kg.The average recoveries of chlorothalonil and 4-hydroxychlorothalonil were 69.3%~111.0% and 65.3%~117.9%,respectively.The relative standard deviations of chlorothalonil and 4-hydroxychlorothalonil were 1.6%~10.8% and 1.1%~9.0%,respectively.［［Conclusion ］］ The method is simple,rapid,sensitive,accurate and reliable for simultaneous determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil in animal -derived food.

Publication Date

2-18-2025

First Page

66

Last Page

72

DOI

10.13652/j.spjx.1003.5788.2024.80531

Recommended Citation

Xiaofang, LIU; Zheng, LI; Hao, HE; Junjie, JIANG; and Yuanyuan, CHEN (2025) "Determination of chlorothalonil and its metabolite 4-hydroxychlorothalonil residues in animal-derived food by ultra-high performance liquid chromatography-tandem mass spectrometry," Food and Machinery: Vol. 40: Iss. 9, Article 10.
DOI: 10.13652/j.spjx.1003.5788.2024.80531
Available at: https://www.ifoodmm.cn/journal/vol40/iss9/10

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