Abstract
［Objective ］ For the residue of propolis after supercritical CO2 extraction,the content of flavonoids in the secondary extraction process was detected in real time.［Methods ］ Near-infrared spectroscopy was used to establish a quantitative analysis model by collecting the spectral information and the corresponding chemical values of flavonoids during the extraction process,which was used to detect the changesof flavonoids content in real time and to determine the end point of extraction.［Results ］ The Rc (corrected correlation coefficient ) and RMSEC (corrected root mean square error ) of the model constructed by the PLS (partial least squares ) algorithm after normalization preprocessing were 0.999 1 and 0.044 5,respectively.The Rp (prediction correlation coefficient ) and RMSEP (the root mean square prediction error ) were 0.929 1 and 0.242 7 respectively,which showed that the in-situ monitoring of the model had good regression and prediction ability.［Conclusion ］ Near-infrared spectroscopy can be used to quickly and accurately monitor the flavonoid content in the extraction process of propolis residue,and realize real-time monitoring in situ.
Publication Date
2-18-2025
First Page
15
Last Page
19,72
DOI
10.13652/j.spjx.1003.5788.2024.80496
Recommended Citation
Wuli, WANG; Haile, MA; Han, LI; Yong, ZHANG; and Hong, XIA
(2025)
"In-situ real-time monitoring of flavonoids extraction from propolis extracts based on near infrared spectroscopy,"
Food and Machinery: Vol. 40:
Iss.
9, Article 2.
DOI: 10.13652/j.spjx.1003.5788.2024.80496
Available at:
https://www.ifoodmm.cn/journal/vol40/iss9/2
References
[1] GIAMPIERI F,QUILES J L,CIANCIOSI D,et al.Bee products:an emblematic example of underutilized sources of |bioactive compounds [J].Journal of Agricultural and Food Chemistry,2022,70(23):6 833-6 848.
[2] 葛 怡 青,汪 浅,仝 涛.蜂 胶 功 能 成 分 及 生 物 活 性 研 究 进 展 [J].食品安全质量检测学报,2022,13(4):1 027-1 035.GE Y Q,WANG Q,TONG T.Research progress on functional components and bioactivities of propolis [J].Journal of Food Safety & Quality,2022,13(4):1 027-1 035.
[3] 王亚群,任永新.蜂胶的化学成分及其保健作用 [J].食品与药品,2006 (12):75-76.WANG Y Q,REN Y X.Chemical components and health effects of propolis [J].Food and Drug,2006 (12):75-76.
[4] BANSKOTA A H,TEZUKA Y,KADOTA S.Recent progress in pharmacological research of propolis [J].Phytother Res,2001,15(7):561-571.
[5] WANG X,WU Q,WU Y,et al.Response surface optimized ultrasonic -assisted extraction of flavonoids from sparganii rhizoma and evaluation of their in vitro antioxidant activities [J].Molecules,2012,17(6):6 769-6 783.
[6] 范埃米,张翠平,卢媛媛,等.蜂胶中的黄酮类化合物 [J].福建农林大学学报 (自然科学版 ),2024,53(1):123-128.FAN A M,ZHANG C P,LU Y Y,et al.Flavonoids in propolis[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition ),2024,53(1):123-128.
[7] CAMPOCCIA D,RAVAIOLI S,SANTI S,et al.Exploring the anticancer effects of standardized extracts of poplar -type propolis:in vitro cytotoxicity toward cancer and normal cell lines[J].Biomedicine & Pharmacotherapy,2021,141(1):111895.
[8] 马海乐,毛丽琴,高寅飞,等.蜂胶超临界 CO2萃取物的体外抑瘤[J].江苏大学学报 (自然科学版 ),2008 (1):5-8.MA H L,MAO L Q,GAO Y F,et al.In vitro inhibitive effect of supercritical CO2 extract of propolis on tumor cells [J].Journal of Jiangsu University (Natural Science Edition ),2008 (1):5-8.
[9] 卢燕珊,彭文君,贲永光.超声提取蜂胶黄酮的研究 [J].声学技术,2020,39(2):190-194.LU Y S,PENG W J,BEN Y G.Ultrasonic extraction of flavonoids from propolis [J].Technical Acoustics,2020,39(2):190-194.
[10] 曾令琴,郭江涛,刘杰,等.芦荟炒制品中总黄酮提取工艺优化及抗氧化活性研究 [J].食品与机械,2023,39(10):175-183.ZENG L Q,GUO J T,LIU J,et al.Optimization of extraction process and antioxidant activity of total flavonoids from Aloe vera stir-fry products [J].Food & Machinery,2023,39(10):175-183.
[11] 尹艳艳,张健,陆伦维,等.刺梨多酚类物质研究进展 [J].食品与机械,2024,40(1):234-240.YIN Y Y,ZHNAG J,LU L W,et al.Research progress on polyphenols in chestnut rose fruit [J].Food & Machinery,2024,40(1):234-240.
[12] 刘孝全,郝经文,陈乃东,等.基于近红外光谱法的定量分析模 型 快 速 预 测 蕨 菜 中 总 黄 酮 的 含 量 [J].理 化 检 验 (化 学 分册),2023,59(11):1 271-1 275.LIU X Q,HAO J W,CHEN N D,et al.Rapid prediction of total flavonoids in pteridium aquilinum by quantitative analysis model based on near infrared spectroscopy [J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis ),2023,59(11):1 271-1 275.
[13] ZHANG Y,LUO L,LI J,et al.In-situ and real -time monitoring of enzymatic process of wheat gluten by miniature fiber NIR spectrometer [J].Food Research International,2017,99:147-154.
[14] 王清.近红外光谱检测技术在农业与食品分析中的应用 [J].智慧农业导刊,2022,2(16):63-65.WANG Q.Application of near infrared spectroscopy in agriculture and food analysis [J].Journal of Smart Agriculture,2022,2(16):63-65.
[15] 孔娜,杨雪,王禹程,等.玛咖多糖提取过程的原位实时监测技术研究 [J].中国食品学报,2019,19(10):235-242.KONG N,YANG X,WANG Y C,et al.Research on in -situ and real -time monitoring technology of polysaccharide extraction from maca [J].Journal of Chinese Institute of Food Science and Technology,2019,19(10):235-242.
[16] 龙若兰,冯丹,罗西,等.藏药五脉绿绒蒿提取过程的在线近红 外 光 谱 质 量 控 制 研 究 [J].分 析 测 试 学 报,2023,42(8):920-929.LONG R L,FENG D,LUO X,et al.Online near infrared quality control on extraction process of tibetan medicine meconopsis quintuplinervia regel [J].Journal of Instrumental Analysis,2023,42(8):920-929.
[17] 张灵枝,黄艳,于英杰,等.基于近红外光谱技术的六大茶类快速识别 [J].食品与生物技术学报,2024,43(1):48-59.ZHANG L Z,HUANG Y,YU Y J,et al.Rapid identification of six major tea categories based on near -infrared spectroscopy[J].Journal of Food Science and Biotechnology,2024,43(1):48-59.
[18] 张雪莉,丁艳华,马海乐,等.茶皂素提取过程的原位实时监测技术 [J].中国食品学报,2022,22(11):326-334.ZHANG X L,DING Y H,MA H L,et al.In-situ and real -time monitoring technology of tea saponin extraction [J].Journal of Chinese Institute of Food Science and Technology,2022,22(11):326-334.
[19] SAMPAIO P N,SALES K C,ROSA F O,et al.In situ near infrared spectroscopy monitoring of cyprosin production by recombinant Saccharomyces cerevisiae strains [J].Journal of Biotechnology,2014,188:148-157.
[20] 卢文杰,卢任杰.紫外分光光度法测定莲子心中总黄酮的含量[J].海峡药学,2020,32(2):83-84.LU W J,LU R J.Determination of total flavonoids in lotus seeds by ultraviolet spectrophotometry [J].Strait Pharmaceutical Journal,2020,32(2):83-84.
[21] 陈静,王宏雁,王宁,等.响应曲面法优化蜂胶中的活性黄酮提取 [J].应用化工,2011,40(10):1 753-1 757.CHEN J,WANG H Y,WANG N,et al.Optimization of extraction of flavonoids from propolis with response surface methodology [J].Applied Chemical Industry,2011,40(10):1 753-1 757.
[22] LUO L,ZHANG Y,WANG K,et al.In situ and real -time monitoring of an ultrasonic -assisted enzymatic hydrolysis process of corn gluten meal by a miniature near infrared spectrometer [J].Analytical Methods,2017,9(25):3 795-3 803.
[23] 李春燕,叶利明,薛金涛.近红外光谱模型快速无损测定肉中 糖 的 含 量 [J].理 化 检 验 (化 学 分 册 ),2023,59(12):1 407-1 412.LI C Y,YE L M,XUE J T.Rapid and nondestructive determination of glucose in meat by near infrared spectroscopy model [J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis ),2023,59(12):1 407-1 412.
[24] 龚润华,杨信廷,郭晓晖,等.基于近红外光谱技术的红小豆品质多指标无损检测及品种鉴别方法研究 [J/OL ].中国粮油学 报.(2024-04-18) [2024-05-20].https://doi.org/10.20048 /j.cnki.issn.1003-0174.000813.GONG R H,YANG X T,GUO X H,et al.Non-destructive determination of multiple quality indicators and varieties of red bean using near -infrared spectroscopy [J/OL ].Journal of the Chinese Cereals and Oils Association.(2024-04-18)[2024-05-20].https://doi.org/10.20048 /j.cnki.issn.1003-0174.000813.
[25] 汤丽华,刘敦华.基于近红外光谱的枸杞化学成分定量分析[J].现代食品科技,2013,29(9):2 306-2 310.TANG L H,LIU D H.Quantitative analysis of lycium barbarum composition based on near infrared spectrum [J].Modern Food Science and Technology,2013,29(9):2 306-2 310.
[26] 蒋 代 余.基 于 深 度 学 习 的 烟 草 近 红 外 光 谱 数 据 分 析 [D].重庆:重庆邮电大学,2021:11-12.JIANG D Y.Analysis of tobacco near infrared spectrum data based on deep learning [D].Chongqing:Chongqing University of Posts and Telecommunications,2021:11-12.
