Abstract
[[Objective ]] This study aimed to develop an efficient extraction process for polysaccharides from southern Shaanxi Polyporus umbellatus using ultrasonic -assisted ionic liquid extraction and evaluate the antioxidant activity of the extract. [Methods ] Use the ultrasonic auxiliary ionic liquid method to extract poronalt polysaccharides,and conduct five -level orthogonal experiments on the basis of single -factor testing.Examine the effects of extracting time,extraction temperature,material liquid ratio and 1-ethyl -3-methylimramidazole acetate quality concentration of the extraction rate of pomollagium polysaccharides.Optimize the process of extracting the poriaon polysaccharide in the liquid liquid of the ultrasonic auxiliary ion liquid.Additionally,the DPPH free radicals clearance and OH free radicals to remove the antioxidant capacity of poronaldium polysaccharides.[Results ] Optimal extraction conditions were achieved with a 60 min ultrasonic time,extraction temperature of 70 ℃,a solid -to-liquid ratio of 1:25 (g/mL ),and a [EMIM ]AC was 3 g/L.Under this condition,the extraction rate of polymal polysaccharides is 8.74%.The extracted polysaccharides showed significant antioxidant activity,effectively scavenging DPPH and OH free radicals.[ConclusionConclusion ] The Ultrasonic -Assisted Ionic Liquid extraction process is efficient and feasible for extracting P.umbellatus polysaccharides,yielding a high extraction rate and strong antioxidant activity.This method enhances the potential application of P.umbellatus polysaccharides as natural antioxidants.
Publication Date
2-18-2025
First Page
167
Last Page
173
DOI
10.13652/j.spjx.1003.5788.2024.80316
Recommended Citation
Miao, ZHU; Zhizhou, LI; Junhai, LIU; and Xiaoyun, LEI
(2025)
"Study on the optimization of the process of extracting Polyporus umbellatus polysaccharides by ultrasonic-assisted ionic liquid and its antioxidant activity,"
Food and Machinery: Vol. 40:
Iss.
9, Article 24.
DOI: 10.13652/j.spjx.1003.5788.2024.80316
Available at:
https://www.ifoodmm.cn/journal/vol40/iss9/24
References
[1] 鲁文静,任慧,崔小敏,等.猪苓利水渗湿的药效物质、药理作用 机 制 及 临 床 应 用 研 究 进 展 [J].中 国 药 房,2023,34(11):1 399-1 403.LU W J,REN H,CUI X M,et al.Research progress on effective components,pharmacological mechanisms and clinical use of Polyporus umbellatus in diuresis-promotion and dampness-clearance [J].Chinese Pharmacy,2023,34(11):1 399-1 403.
[2] 王天媛,张飞飞,任跃英,等.猪苓化学成分及药理作用研究进展 [J].上海中医药杂志,2017,51(4):109-112.WANG T Y,ZHANG F F,REN Y Y,et al.Research progress in study on chemical constituents and pharmacological effects of Polyporus umbellatus [J].Shanghai Traditional Chinese Medicine Magazine,2017,51(4):109-112.
[3] 赖戈娜,贾文玉,罗思婉,等.猪苓多糖的 PMP 柱前衍生化 -HPLC指纹图谱研究 [J].中国药房,2020,31(7):788-793.LAI G N,JIA W Y,LUO S W,et al.Study on pre-column derivatization HPLC finger print of Polyporus umbellatus [J].Chinese Pharmacy,2020,31(7):788-793.[4] LUO S W,HUANG X P,LI S Q,et al.Homogeneous Polyporus polysaccharide exerts anti-bladder cancer effects via autophagy induction [J].Pharmaceutical Biology,2024,62(1):214-221.
[5] JIA W Y,LUO S W,LAI G N,et al.Homogeneous Polyporus polysaccharide inhibits bladder cancer by polarizing macrophages to M 1 subtype in tumor microenvironment [J].BMC Complementary Medicine and Therapies,2021,21(1):150.
[6] 霍达,张竞男,马微,等.响应面法优化内蒙古肉苁蓉多糖提取工艺 [J].世界中医药,2023,18(12):1 667-1 671.HUO D,ZHANG J N,MA W,et al.Optimization of extraction process of Cistanche deserticola polysaccharide form Leimengguo by response surface methodology [J].World Chinese Medicine,2023,18(12):1 667-1 671.
[7] 王晨曦,夏依旦·买合苏木,房徐涵,等.芜菁子油脂微波提取工 艺 及 其 脂 肪 酸 和 生 物 活 性 研 究 [J].粮 食 与 油 脂,2023,36(10):56-62.WANG C X,MAHSUM X,FANG X H,et al.Study on microwave extraction technology of Brassica rapa L.seed oil and its fatty acids and biological activity [J].Grain and Oil,2023,36(10):56-62.
[8] 陶艳阳,吴凯,赵生文.超声辅助复合酶法对冬枣多糖的影响研究 [J].食品科技,2023,48(4):208-216.TAO Y Y,WU K,ZHAO S W.Effect of ultrasonic assisted compound enzyme method on Zizyphus jujube Cv.Dongzao ploysaccharides [J].Food Technology,2023,48(4):208-216.
[9] 韩晓磊,吕慧英,梁珏钦,等.春生田头菇粗多糖超声辅助提取及抗氧化性测定 [J].食品与机械,2023,39(9):169-176.HAN X L,LU H Y,LIANG Y Q,et al.Ultrasound-assisted extraction and antioxidant study of crude polysaccharide from Agrocybe praecox [J].Food & Machinery,2023,39(9):169-176.
[10] MAGNIM J P,PAPAICONOMOU N,BILLARD I.Polyphenol,polysaccharide and lactate extraction from pulping factory black liquor by ionic liquids [J].Separation and Purification Technology,2018,196:140-148.
[11] DONG Q Q,QIU W Q,LI L,et al.Extraction of chitin from white shrimp (Penaeus vannamei ) shells using binary ionic liquid mixtures [J].Journal of Industrial and Engineering Chemistry,2023,120:529-541.
[12] LI D,GAO Y M,MU M Y,et al.Ionic liquid-modified UiO-66-NH2 as sorbent of dispersive solid-phase extraction for rapid adsorption and enrichment of benzoylurea insecticides[J].Microchimica Acta,2023,190(11):446.
[13] CHEN W W,WU Y C,HE J,et al.Ionic liquids assisted construction of efficient ceramic-based catalyst by direct ink writing 3D printing for ultra-deep oxidative desulfurization of diesel [J].Ceramics International,2024,50(7):10 990-11 002.
[14] SETAREH S,ALIREZA H J.Ionic liquids in green energy storage devices:lithium-ion batteries,supercapacitors,and |solar cells [J].Monatshefte für Chemie - Chemical Monthly,2024,155(5):383-399.
[15] FAN H,GAO J,ZHANG B,et al.Effects of modified alsub 2/subOsub 3/sub-decorated ionic liquid on the mechanical properties and impact resistance of a polyurethane elastomer[J].Materials,2021,14(16):4 712.
[16] DANG M,DENG Q L,FANG G Z,et al.Preparation of novel anionic polymeric ionic liquid materials and their potential application to protein adsorption [J].Journal of Materials Chemistry B,2017,5(31):6 339-6 347.
[17] 王彦平,娄芳慧,陈月英,等.苯酚—硫酸法测定紫山药多糖含量的条件优化 [J].食品研究与开发,2021,42(4):170-174.WANG Y P,LOU F H,CHEN Y Y,et al.Optimization of analytical conditions for the determination of polysaccharides contents in purple yam by phenol-sulfuric acid method [J].Food Research and Development,2021,42(4):170-174.
[18] 周加林,张涵,岳义民,等.响应面法优化地黄多糖的提取工艺 及 抗 氧 化 活 性 分 析 [J].广 东 药 科 大 学 学 报,2022,38(4):64-71.ZHOU J L,ZHANG H,YUE Y M,et al.Optimization of extraction and antioxidant activity of crude polysaccharides from Rehmannia glutinosa Libosh.by response surface methodology [J].Journal of Guangdong Pharmaceutical University,2022,38(4):64-71.
[19] 唐森,梁冰娜,覃逸明,等.响应面优化白芨水溶性多糖提取工艺的研究 [J].山东化工,2019,48(22):3-7.TANG S,LIANG B N,QIN Y M,et al.Extraction process of polysaccharide in Bletilla striata optimized by response surface method [J].Shandong Chemical,2019,48(22):3-7.
[20] 张璐.酸枣果肉多糖的提取优化及抗氧化活性研究 [J].中国酿造,2015,34(8):92-96.ZHANG L.Optimization of extraction technology of polysaccharide from sour jujube fruits and its antioxidant activity [J].Chinese Brewing,2015,34(8):92-96.
[21] HUMA S,HANAN S,AMIR S,et al.Assessment of antioxidant activity of pure graphene oxide (GO) and composite V2O5/GO using DPPH radical and H2O2 scavenging assays [J].Journal of Sol-Gel Science and Technology,2023,108(3):840-849.
[22] 张小霞,吴巧凤,董旭旦,等.猪苓多糖的提取工艺优化及抗氧化活性研究 [J].中华中医药学刊,2016,34(8):2 025-2 028.ZHANG X X,WU Q F,DONG X D,et al.Optimizing extraction process of Polyporus umbellatus polysaccharide and its anti-oxidation activity [J].Chinese Chinese Medicine Journal,2016,34(8):2 025-2 028.
[23] 郑伊琦,张安强,张小军,等.响应面优化猪苓菌核多糖超声辅 助 提 取 工 艺 及 抗 氧 化 活 性 分 析 [J].食 品 工 业 科 技,2023,44(16):255-263.ZHENG Y Q,ZHANG A Q,ZHANG X J,et al.Optimization of ultrasonic-assisted extraction of polysaccharides from Polyporus umbellatus sclerotium by response surface methodology and antioxidant activity [J].Food Industry Technology,2023,44(16):255-263.
[24] 刘国库.国内主产区猪苓指纹图谱特征及菌丝体胞外多糖活性研究 [D].咸阳:西北农林科技大学,2020:155-157.LIU G K.Fingerprint characteristics Polyporus umbellatus from main producing areas in China and exopolysaccharide activities from the mycelium [D].Xianyang:Northwestern Agricultural and Forestry University of Science and Technology,2020:155-157.
[25] 张静宇,于京,姜丽影,等.猪苓多糖口服液的制备及其抗氧化性的初步研究 [J].云南化工,2018,45(8):9-11.ZHANG J Y,YU J,JIANG L Y,et al.Preparation of Polyporus polysaccharide oral liquid and preliminary study on its antioxidant activity [J].Yunnan Chemical,2018,45(8):9-11.
[26] 张元,冯琼,杨晓方,等.酶解—超声法对猪苓多糖正交优选提 取 及 抗 氧 化 活 性 的 初 步 实 验 研 究 [J].天 然 产 物 研 究 与 开发,2015,27(9):1 657-1 662,1 570.ZHANG Y,FENG Q,YANG X F,et al.Optimization of enzymatic coupled with ultrasonic-assisted extraction of polysaccharide from Polyporus umbellatus and its antioxidant activity [J].Natural Product Research and Development,2015,27(9):1 657-1 662,1 570.
[27] 陈凌,陈召桂,骆卢佳,等.不同方法提取马齿苋多糖的抗氧活性比较 [J].食品研究与开发,2015,36(22):14-18.CHEN L,CHEN Z G,LUO L J,et al.The comparison of antioxidant activity of polysaccharides extracted from purslane by different ways [J].Food Research and Development,2015,36(22):14-18.