Detection of allergen mango components by using droplet digital PCR technology

Authors

LI Enjing, Beijing Institute of Food Inspection and Research (Beijing Municipal Center for Food Safety Monitoring and Risk Assessment , Beijing 100094 , China ;Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation , Beijing 100094 , ChinaFollow
WANG Dan, Beijing Institute of Food Inspection and Research (Beijing Municipal Center for Food Safety Monitoring and Risk Assessment , Beijing 100094 , China ;Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation , Beijing 100094 , China
LI Long, Beijing Institute of Food Inspection and Research (Beijing Municipal Center for Food Safety Monitoring and Risk Assessment , Beijing 100094 , China ;Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation , Beijing 100094 , China
LI Hejing, Beijing Institute of Food Inspection and Research (Beijing Municipal Center for Food Safety Monitoring and Risk Assessment , Beijing 100094 , China ;Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation , Beijing 100094 , China
YANG Honglian, Beijing Institute of Food Inspection and Research (Beijing Municipal Center for Food Safety Monitoring and Risk Assessment , Beijing 100094 , China ;Laboratory of Key Technologies of Major Comprehensive Guarantee of Food Safety for State Market Regulation , Beijing 100094 , China

Corresponding Author(s)

李恩静（1983—），女，北京市食品检验研究院工程师，硕士。E-mail：enjingli@126.com

Abstract

［［Objective ］］ A droplet digital PCR method for detect allergen mango components was established in this study.[Methods ] Firstly,the chloroplast trnH-psbA gene was selected as the target gene for detect allergen mango components.Secondly,specific primers and probe were designed.Finally,the reaction conditions of droplet digital PCR was optimized to verify the sensitivity and stability of the method.[Results ] This method had been applied to detect allergen mango components in 10 batches of food samples.The results of establishment of droplet digital PCR method was showed good specificity and not cross -react with 10 kinds of other fruits except mango.Under the optimal ddPCR reaction conditions,which were to have the concentration of forward and reverse primers and probe set as 200,200 and 100 nmol/L,respectively,and to have annealing temperature set as 52 ℃,the detection sensitivity of nucleic acid level reached 5 pg/μL,and the detection sensitivity of mango content reached 5 mg/kg,the coefficient of variation of the method was less than 25%.The test results of 10 batches of food samples applied with the method were consistent with the food labels.[Conclusion ] This method has the advantages of being fast,specific,and highly sensitive,making it suitable for the detection of allergenic mango components.

Publication Date

2-18-2025

First Page

50

Last Page

55,115

DOI

10.13652/j.spjx.1003.5788.2023.81139

Recommended Citation

Enjing, LI; Dan, WANG; Long, LI; Hejing, LI; and Honglian, YANG (2025) "Detection of allergen mango components by using droplet digital PCR technology," Food and Machinery: Vol. 40: Iss. 9, Article 7.
DOI: 10.13652/j.spjx.1003.5788.2023.81139
Available at: https://www.ifoodmm.cn/journal/vol40/iss9/7

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