Abstract
［Objective］ To establish a new method for simultaneous detection of quinolones (QNs ) and sulfonamides (SAs) in aquatic products and livestock products.［Methods］ In pretreatment,this work compares the effect of extraction solvents on the recoveries and investigates the reduction or elimination effect of purification methods such as liquid -liquid extraction (n-hexane ),dispersive adsorption of the matrix (C18 and ethylenediamine -N-propyl silylated silica ),and general solid phase extraction (PRiME HLB ) on the matrix effect.The sample is pretreated by acetonitrile with 1.0% formic acid,purified by EMR -Lipid,and separated by ACQUITY UPLC®HSST3 column (3.5 µm,2.1 mm ×100 mm ) in 10 min.MS detection is performed by multi -reaction monitoring (MRM ) in positive ion mode.QNs and SAs are quantified by the external standard method.［Results］ The calibration curves of QNs and SAs exhibit a good linear relationship with the correlation coefficients (R2) exceeding 0.99,in the range of 1.0~100.0 ng/mL.The limits of detection (LODs ) and quantification (LOQs ) are 1.0 and 2.0 μg/kg,respectively.The average recoveries of QNs and SAs are between 61.9% and 115.3% under low,middle,and high spiked levels and the relative standard deviations (RSDs ) are less than 15% (n=6).［Conclusion］ Compared with literature and standard methods,the established method increases the scope of application and reduces the pre -treatment process and cost.
Publication Date
4-3-2025
First Page
63
Last Page
73
DOI
10.13652/j.spjx.1003.5788.2024.80456
Recommended Citation
Yu, QIN; Feng, XUE; Junjie, YU; Yang, LIU; and Heng, GONG
(2025)
"Determination of quinolones and sulfonamides residues in aquatic products and livestock products with enhanced matrix removal-lipid adsorbent by liquid chromatography-tandem mass spectrometry,"
Food and Machinery: Vol. 41:
Iss.
1, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2024.80456
Available at:
https://www.ifoodmm.cn/journal/vol41/iss1/8
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