Abstract
［Objective］ To explore the feasibility of cultivating Schizochytrium sp.using waste beer yeast hydrolysate to produce DHA algal oil.［Methods］ Ultrasound -assisted composite enzyme method was selected to break the cell wall and hydrolyze waste beer yeast,optimizing the process based on reducing sugar and total nitrogen concentrations as response variables.The hydrolysate was then used to cultivate Schizochytrium sp.to produce DHA algal oil.［Results］ Under the conditions of ultrasonic power at 400 W,temperature at 60 ℃,pH 5.0,papain at 0.5 g,β-glucanase:papain ratio of 4:5,and hydrolysis time of 40 min,the hydrolysate achieved maximum concentrations of reducing sugar and total nitrogen,which were 20.40 g/L and 6.72 g/L,respectively.When the hydrolysate volume fraction was 75% and the inoculum volume fraction of Schizochytrium sp.was 14%,after 3 days of cultivation,the biomass,oil yield,DHA content,and DHA yield reached their maximum values of 17.14 g/L,2.88 g/L,3.13%,and 0.55 g/L,respectively,which were higher than those obtained from traditional fermentation cultures (biomass 10.29 g/L,oil yield 1.84 g/L,DHA content 2.76%,DHA yield 0.29 g/L).The pathogenic bacteria and heavy metal levels in the algal oil were below the minimum limits specified in national food safety standard.［Conclusion］ Using waste beer yeast hydrolysate obtained through ultrasound -assisted composite enzyme hydrolysis to cultivate Schizochytrium sp.for DHA production is feasible.This method achieves an organic integration of food production waste treatment and microalgae -based chemical product production.
Publication Date
4-3-2025
First Page
173
Last Page
180
DOI
10.13652/j.spjx.1003.5788.2024.80177
Recommended Citation
Qingyi, YANG; Yufang, TANG; Tao, LI; Lei, LIU; and Dake, ZHOU
(2025)
"Production of DHA algal oil from Schi zochytrium sp. fed with waste beer yeast hydrolysate,"
Food and Machinery: Vol. 41:
Iss.
2, Article 22.
DOI: 10.13652/j.spjx.1003.5788.2024.80177
Available at:
https://www.ifoodmm.cn/journal/vol41/iss2/22
References
[1] GUO D S,JI X J,REN L J,et al.Development of a multi-stage continuous fermentation strategy for docosahexaenoic acid production by Schizochytrium sp.[J].Bioresource Technology,2018,269:32-39.
[2] KUJAWSKA N,TALBIERZ S,DĘBOWSKI M,et al.Cultivation method effect on Schizochytrium sp.biomass growth and docosahexaenoic acid (DHA ) production with the use of waste glycerol as a source of organic carbon [J].Energies,2021,14(10):2 952.
[3] WANG S K,WANG X,TIAN Y T,et al.Nutrient recovery from tofu whey wastewater for the economical production of docosahexaenoic acid by Schizochytrium sp.S31[J].Science of the Total Environment,2020,710:136448.
[4] ESTÉVEZ A,PADRELL L,IÑARRA B,et al.Brewery by-products (yeast and spent grain ) as protein sources in gilthead seabream (Sparus aurata ) feeds [J].Aquaculture,2021,543:736921.
[5] 董爱华.啤酒废酵母水解物的生产工艺优化及其在仔猪生产中的应用 [D].广州:华南理工大学,2019:1-6.DONG A H.Optimization of production process of brewer's yeast hydrolysate and its application in pig production [D].Guangzhou:South China University of Technology,2019:1-6.
[6] LIN D H,LOPEZ-SANCHEZ P,LI R,et al.Production of bacterial cellulose by Gluconacetobacter hansenii CGMCC 3917 using only waste beer yeast as nutrient source [J].Bioresource Technology,2014,151:113-119.
[7] 黄海.啤酒废酵母多糖及谷胱甘肽提取工艺的研究 [D].兰州:兰州理工大学,2018:9-20.HUANG H.Study on the extraction process of polysaccharides and glutathione from waste beer yeast [D].Lanzhou:Lanzhou University of Technology,2018:9-20.
[8] THANH H V,PHI N T L,KHOI N T,et al.Green extraction and biological activity of phenolic extracts from cashew nut testa using a combination of enzyme and ultrasound-assisted treatments [J].Journal of the Science of Food and Agriculture,2023,103(11):5 626-5 633.
[9] ZHANG C,LI J J,CHEN L,et al.Effects of alkali,enzymes,and ultrasound on monosodium glutamate byproduct for a sustainable production of Bacillus subtilis [J].Food Chemistry,2021,360:129967.
[10] 张莉弘,吴琼.几种废啤酒酵母细胞破壁方法的比较 [J].长春大学学报,2014,24(4):464-467.ZHANG L H,WU Q.comparison of a few kinds of cell-wall breaking methods for waste beer yeast [J].Journal of Changchun University,2014,24(4):464-467.
[11] SZABO O E,CSISZAR E.Some factors affecting efficiency of the ultrasound-aided enzymatic hydrolysis of cotton cellulose [J].Carbohydrate Polymers,2017,156:357-363.
[12] YUAN H J,HE Y,ZHANG H,et al.Ultrasound-assisted enzymatic hydrolysis of yeast β-glucan catalyzed by β-glucanase:chemical and microstructural analysis [J].Ultrasonics Sonochemistry,2022,86:106012.
[13] 唐裕芳,刘磊,杨雨欣,等.胺鲜酯和 2,4-二氯苯氧乙酸协同诱 导 裂 殖 壶 菌 积 累 DHA [J].化 工 进 展,2023,42(11):5 900-5 907.TANG Y F,LIU L,YANG Y X,et al.Synergy inducting effect of diethyl aminoethyl hexanoate and 2,4-dichlorophenoxyacetic acid on DHA accumulation in Schizochytrium sp.[J].Chemical Industry and Engineering Progress,2023,42(11):5 900-5 907.
[14] 周志豪,李芸芸,王唯,等.酱油渣水解液养殖小球藻积累蛋白 质 可 行 性 研 究 [J].湘 潭 大 学 学 报 (自 然 科 学 版 ),2023,45(1):115-126.ZHOU Z H,LI Y Y,WANG W,et al.Feasibility of cultivation of Chlorella protothecoides in soy sauce residue hydrolysate and protein accumulation [J].Journal of Xiangtan University (Natural Science Edition ),2023,45(1):115-126.
[15] 张剑,杨雨欣,唐裕芳,等.六种植物生长调节剂促进裂壶藻油 脂 和 DHA 积 累 的 研 究 [J].湘 潭 大 学 学 报 (自 然 科 学 版 ),2022,44(4):81-89.ZHANG J,YANG Y X,TANG Y F,et al.Investigation of six plant growth regulators promoting lipid and DHA accumulation in Schizochytrium [J].Journal of Xiangtan University (Natural Science Edition ),2022,44(4):81-89.
[16] CAO Z H,GUO Y,LIU Z H,et al.Ultrasonic enzyme-assisted extraction of comfrey (Symphytum officinale L.) polysaccharides and their digestion and fermentation behaviors in vitro [J].Process Biochemistry,2022,112:98-111.
[17] YUN C,WANG S F,GAO Y,et al.Optimization of ultrasound-assisted enzymatic pretreatment for enhanced extraction of baicalein and wogonin from Scutellaria baicalensis roots [J].Journal of Chromatography B,2022,1 188:123077.
[18] LADOLE M R,NAIR R R,BHUTADA Y D,et al.Synergistic effect of ultrasonication and co-immobilized enzymes on tomato peels for lycopene extraction [J].Ultrasonics Sonochemistry,2018,48:453-462.
[19] YUAN H J,HE Y,ZHANG H,et al.Ultrasound-assisted enzymatic hydrolysis of yeast β-glucan catalyzed by β-glucanase:chemical and microstructural analysis [J].Ultrasonics Sonochemistry,2022,86:106012.
[20] QU Y B X,YANG Z G,LI H P,et al.Ultrasound-assisted enzymatic extraction method for multi-element analysis of rice[J].Food Analytical Methods,2020,13(8):1 549-1 555.
[21] 罗轩.蜂花粉多肽的超声辅助酶解制备及其过程原位实时监测技术研究 [D].苏州:江苏大学,2022:32-46.LUO X.Ultrasound-assisted enzymatic preparation of bee pollen peptides and in-situ real-time monitoring of the process[D].Suzhou:Jiangsu University,2022:32-46.[22] 徐康,王远兴.半固态酶解法制备豆粕肽工艺的优化 [J].中国食品学报,2023,23(7):278-288.XU K,WANG X Y.Optimization of preparation of soybean meal peptides by semi-solid enzymatic hydrolysis [J].Journal of Chinese Institute of Food Science and Technology,2023,23(7):278-288.
[23] 陈灼娟,柯秀贤,黄霄.姬松茸抗氧化酶解液的制备 [J].食品与机械,2023,39(3):183-187,232.CHEN Z J,KE X X,HUANG X.Preparation of antioxidant hydrolysate of Agaricus blazei Murill [J].Food & Machinery,2023,39(3):183-187,232.
[24] HOAI T V,CAO P N,LE THAO M P,et al.Ultrasound-assisted enzymatic extraction of adenosine from vietnamese cordyceps militaris and bioactivity analysis of the extract [J].Journal of Chemistry,2020,2 020:1487654.
[25] BAO Z D,ZHU Y M,ZHANG K,et al.High-value utilization of the waste hydrolysate of Dioscorea zingiberensis for docosahexaenoic acid production in Schizochytrium sp.[J].Bioresource Technology,2021,336:125305.
[26] BAI Y N,LI Y Q,TANG Y F,et al.Rhizopus oryzae fermentation wastewater nutrient removal coupling protein fodder production by employing Chlorella pyrenoidosa [J].Bioresource Technology,2022,362:127858.
[27] 秦宇,欧莹,王尤婧,等.利用酒糟处理液作为氮源培养裂殖壶 菌 生 产 DHA 的 发 酵 工 艺 优 化 [J].中 国 油 脂,2023,48(3):116-122.QIN Y,OU Y,WANG Y J,et al.Optimization of fermentation process of culturing Schizochytrium to produce DHA using distiller's grains treatment liquid as nitrogen source [J].China Oils and Fats,2023,48(3):116-122.
