Abstract
［Objective］ In this study,an optimized strategy was proposed to address the limitations of current methods for detecting selenium species in selenium -enriched edible oils,such as the transformation between selenium species,long enzymatic hydrolysis time,and the limited number of detectable selenium species.［Methods］ Protease K -assisted hydrolysis was performed using accelerated solvent extraction,and a high performance liquid chromatography -inductively coupled plasma mass spectrometry (HPLC -ICP-MS) method was established.［Results］ At 40 ℃,with a proteinase K dosage of 35 mg and a static extraction time of 5 min,the extraction rate of selenium species could reach a maximum of (83.65±2.36)%.Eight target selenium species could be effectively separated within 20 min,with good linearity in the range of 2~100 μg/L (R2≥0.999 3),detection limits of 0.20~0.60 μg/kg,quantification limits of 0.60~1.80 μg/kg,spiked recovery rates between 82.3% and 112.3%,and relative standard deviations ≤5.60%.［Conclusion］ This method improves the efficiency and accuracy of selenium speciation analysis.
Publication Date
4-3-2025
First Page
56
Last Page
64
DOI
10.13652/j.spjx.1003.5788.2024.81239
Recommended Citation
Zhisen, LIANG; Shudi, ZHANG; Xiao, LING; and Yuzhen, CHEN
(2025)
"Determination of eight selenium species in selenium-enriched edible oil with accelerated solvent extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry,"
Food and Machinery: Vol. 41:
Iss.
2, Article 7.
DOI: 10.13652/j.spjx.1003.5788.2024.81239
Available at:
https://www.ifoodmm.cn/journal/vol41/iss2/7
References
[1] 冯灏,苏莹,耿雅雯,等.HPLC-ICP-MS 同时测定富硒食品中砷形态和硒形态 [J].食品与机械,2024,40(7):43-52.FENG H,SU Y,GENG Y W,et al.Study on simultaneous determination of arsenic speciation and selenium speciation in selenium-rich foods by HPLG-ICP-MS [J].Food & Machinery,2024,40(7):43-52.
[2] LV Q Z,LIANG X M,NONG K Y,et al.Advances in research |on the toxicological effects of selenium [J].Bulletin of Environmental Contamination and Toxicology,2021,106(5):715-726.
[3] NIE X L,YANG X R,HE J Y,et al.Bioconversion of inorganic selenium to less toxic selenium forms by microbes:a review [J].Front Bioeng Biotechnol,2023,11:1167123.
[4] 刘为,尹金晶,吴慕慈,等.富硒农产品中硒代氨基酸形态及其在不同蛋白组分中的分布 [J].食品与机械,2022,38(6):45-51,190.LIU W,YI J J,WU M C,et al.Selenium amino acids speciation in selenium-enriched agricultural products and their distribution in different protein components [J].Food & Machinery,2022,38(6):45-51,190.
[5] BUCHNER P,TAKAHASHI H,HAWKESFORD M J.Plant sulphate transporters:co-ordination of uptake,intracellular and long-distance transport [J].Journal of Experimental Botany,2004,55(404):1 765-1 773.
[6] OUERDANE L,BOTH E B,XIANG J Q,et al.Water soluble selenometabolome of Cardamine violifolia [J].Metallomics,2020,12(12):2 032-2 048.
[7] BOTH E B,SHAO S X,XIANG J Q,et al.Selenolanthionine is the major water-soluble selenium compound in the selenium tolerant plant Cardamine violifolia [J].Biochimica et Biophysica Acta (BBA )-General Subjects,2018,1 862(11):2 354-2 362.
[8] ABDULAH R,MIYAZAKI K,NAKAZAWA M,et al.Chemical forms of selenium for cancer prevention [J].Journal of Trace Elements in Medicine and Biology,2005,19(2/3):141-150.
[9] CAI J,SUN W,HWANG J,et al.Changes in S-adenosylmethionine synthetase in human liver cancer:molecular characterization and significance [J].Hepatology,1996,24(5):1 090-1 097.
[10] XIONG Y Y,HUANG Y T,LI L,et al.A review of plant selenium-enriched proteins/peptides:extraction,detection,bioavailability,and effects of processing [J].Molecules,2023,28(3):1 223.
[11] GERGELY V,KUBACHKA K M,MOUNICOU S,et al.Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry [J].Journal of Chromatography A,2006,1 101(1/2):94-102.
[12] 陈冰冰,杨奕,李嘉颐,等.富硒辣木籽蛋白降压肽的酶法制备、硒 含 量 及 稳 定 性 研 究 [J].食 品 与 机 械,2022,38(8):213-221.CHEN B B,YANG Y,LI J Y,et al.Preparation,selenium content and stability of antihypertensive peptides from selenium-enriched Moringa oleifera seed protein by enzymatic hydrolysis [J].Food & Machinery,2022,38(8):213-221.
[13] WU M L,ZHU Z Z,LI S Y,et al.Green recovery of Se-rich protein and antioxidant peptides from Cardamine violifolia:composition and bioactivity [J].Food Bioscience,2020,38:100743.
[14] 梁志森,陈玉珍,李建华,等.高效液相色谱—电感耦合等离子体质谱法同时测定植物性富硒食品中 7种硒元素形态含量[J].食品安全质量检测学报,2024,15(9):142-150.LIANG Z S,CHEN Y Z,LI J H,et al.Simultaneously determination of the content of 7 kinds of selenium species in plant-based selenium-enriched food by high performance liquid chromatography-inductively coupled plasma mass spectrometry [J].Journal of Food Safety & Quality,2024,15(9):142-150.
[15] 王禹衡,卢思佳,张婧文,等.快速溶剂萃取—离子色谱—质谱 法 同 时 测 定 爆 炸 尘 土 中 的 氯 酸 盐 和 高 氯 酸 盐 [J].分 析 试验室,2023,42(4):474-479.WANG Y H,LU S J,ZHANG Q W,et al.Simultaneous determination of chlorate and perchlorate in explosion dust by accelerated solvent extraction-ion chromatography-mass spectrometry [J].Chinese Journal of Analysis Laboratory,2023,42(4):474-479.
[16] TIE M,LI B R,SUN T B,et al.HPLC-ICP-MS speciation of selenium in Se-cultivated Flammulina velutipes [J].Arabian Journal of Chemistry,2020,13(1):416-422.
[17] CHEN B B,HU B,HE M,et al.Speciation of selenium in cells by HPLC-ICP-MS after (on-chip ) magnetic solid phase extraction [J].Journal of Analytical Atomic Spectrometry,2013,28(3):334-343.
[18] 杨芬,谢邵文,韦朝阳,等.HPLC-ICP-MS 联用技术研究甲醇对 砷 形 态 分 析 结 果 的 影 响 [J].光 谱 学 与 光 谱 分 析,2018,38(6):1 884.YANG F,XIE S W,WEI C Y,et al.Effects of methanol addition on arsenic speciation analysis with HPLC-ICP-MS [J].Spectroscopy and Spectral Analysis,2018,38(6):1884.
[19] 刘 文 政,贾 亚 琪,周 贻 兵,等.超 声 提 取 结 合 HPLC-ICP-MS联 用 测 定 富 硒 山 茶 油 中 的 硒 形 态 [J].中 国 粮 油 学 报,2023,38(9):197-203.LIU W Z,JIA Y Q,ZHOU Y B,et al.Determination of selenium species in selenium enriched Camellia oil by ultrasonic extraction combined with HPLC-ICP-MS [J].Journal of the Chinese Cereals and Oils Association,2023,38(9):197-203.
[20] 韦淑毅,钟其顶,高红波,等.高效液相色谱法检测富硒酵母中的硒代蛋氨酸 [J].食品研究与开发,2012,33(8):164-168.WEI S Y,ZHONG Q D,GAO H B,et al.Determination of selenomethionine in selenium-enriched yeast by reversed phase high performance liquid chromatography [J].Food Research and Development,2012,33(8):164-168.
[21] 丁健桦,徐文平,朱玉玲.混合模式固相萃取—高效液相色谱法测定牛奶中 4种非甾体抗炎药残留 [J].分析化学,2023,51(6):1 013-1 023.DING J H,XU W P,ZHU Y L.Mixed-mode solid phase extraction coupled with high performance liquid chromatography for analysis of non-steroidal anti-inflammatory drugs residues in Bovine milk [J].Chinese Journal of Analytical Chemistry,2023,51(6):1 013-1 023.
[22] 王 纪,廖 海 明,范 慧 红.混 合 模 式 色 谱 -CAD-LTQ Orbitrap MS联用分析甲硫氨酸中的杂质 [J].中国药学杂志,2021,56(22):1 824-1 828.WANG J,LIAO H M,FAN H H.Analysis of impurity in methionine by mixed-mode chromatography-CAD-LTQ orbitrap MS [J].Chinese Pharmaceutical Journal,2021,56(22):1 824-1 828.
[23] CAO J P,CHENG Y Z,XU B C,et al.Determination of different selenium species in selenium-enriched polysaccharide by HPLC-ICP-MS [J].Food Analytical Methods,2021,14(11):2 420-2 429.
[24] KOTREBAI M,TYSON J F,BLOCK E,et al.High-performance liquid chromatography of selenium compounds utilizing perfluorinated carboxylic acid ion-pairing agents and inductively coupled plasma and electrospray ionization mass spectrometric detection [J].Journal of Chromatography A,2000,866(1):51-63.
[25] ZAINAL H A,LACROIX D E,WOLF W R.Utilization of chromatographic and spectroscopic techniques to study the oxidation kinetics of selenomethionine [J].Analytical and Bioanalytical Chemistry,1996,356(5):311-314.
[26] DIEMER V,OLLIVIER N,LECLERCQ B,et al.A cysteine selenosulfide redox switch for protein chemical synthesis [J].Nature Communications,2020,11(1):2 558.
[27] PEHLIVAN Ö,WOJTKOWIAK K,JEZIERSKA A,et al.Photochemical transformations of peptides containing the N-(2-selenoethyl )glycine moiety [J].ACS Omega,2024,9(14):16 775-16 791.
[28] 赵小红,何慧,贾蕾,等.生物强化硒和锌的豆芽制备、分布及形态研究 [J].食品科技,2022,47(1):70-78.ZHAO X H,HE H,JIA L,et al.Study on preparation,distribution and morphology of bean sprouts biologically fortified with selenium and zinc [J].Food Science and Technology,2022,47(1):70-78.
[29] 何潇潇,周健,马懿,等.加工方式对富硒菜籽油中硒含量及形态变化的影响 [J].现代食品,2023,29(17):200-206.HE X X,ZHOU J,MA Y,et al.Effects of processing methods on selenium content and form changes in selenium-enriched rapeseed oil [J].Modern Food,2023,29(17):200-206.
[30] DUMONT E,VANHAECKE F,CORNELIS R.Selenium speciation from food source to metabolites:a critical review[J].Analytical and Bioanalytical Chemistry,2006,385(7):1 304-1 323.
[31] 吴雅颖,桂仁意,汤鋆,等.HPLC-ICP-MS 联用技术测定竹笋中六种硒形态 [J].营养学报,2014,36(5):494-498.WU Y Y,GUI R Y,TANG J,et al.Analysis of six selenium species in bamboo shoot by high performance liquid chromatography combined with inductively coupled plasma mass spectrometry [J].Acta Nutrimenta Sinica,2014,36(5):494-498.
[32] 倪策,曹天红,陈敏,等.核桃粕源抗氧化活性肽的酶解制备及活性分析 [J].食品与机械,2024,40(5):51-61.NI C,CAO T H,CHEN M,et al.Enzymatic hydrolysis preparation and activity analysis of antioxidant peptides derived from walnut dregs [J].Food & Machinery,2024,40(5):51-61.
[33] 季海冰,章佳,刘莉.快速溶剂萃取—高效液相色谱—电感耦合等离子体质谱法快速测定淀粉类农作物中 4种形态硒 [J].理化检验 (化学分册 ),2020,56(9):979-983.JI H B,ZHANG J,LIU L.HPLC-ICP-MS rapid determination of 4 species of selenium in starchy crops with accelerated solvent extraction [J].Physical Testing and Chemical Analysis (Part B:Chemical Analysis ),2020,56(9):979-983.
[34] 孟莉,许亚丽,夏曾润,等.高效液相色谱—电感耦合等离子体 质 谱 法 测 定 谷 类 食 品 中 的 4种 硒 形 态 [J].分 析 科 学 学 报,2021,37(6):843-846.MENG L,XU Y L,XIA Z R,et al.Determination of selenium species in cereals by high performance liquid chromatography-inductively coupled plasma-mass spectrometry [J].Journal of Analytical Science,2021,37(6):843-846.
