Quantification of eight biogenic amines in aquatic animal products with amino acid analyzer

Authors

FENG Hongyan, Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology , Lianyungang , Jiangsu 222000 , China ;Seafood Quality Supervision and Inspection Center of Jiangsu Province , Lianyungang , Jiangsu 222000 , China
XIA Yongsheng, Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology , Lianyungang , Jiangsu 222000 , China
QIAN Liangliang, Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology , Lianyungang , Jiangsu 222000 , China ;Seafood Quality Supervision and Inspection Center of Jiangsu Province , Lianyungang , Jiangsu 222000 , China
CHEN Xiaojuan, Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology , Lianyungang , Jiangsu 222000 , China
ZHOU Jing, Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology , Lianyungang , Jiangsu 222000 , China ;Seafood Quality Supervision and Inspection Center of Jiangsu Province , Lianyungang , Jiangsu 222000 , ChinaFollow

Corresponding Author(s)

周静（1970—），女，连云港市质量技术综合检验检测中心正高级工程师，硕士。E-mail：zhoujing158@163.com

Abstract

［Objective］ To address the cumbersome manual pre -column derivatization steps and poor derivative stability encountered in traditional chromatography when determining biogenic amines,so as to improve the efficiency and accuracy of detection.［Methods］ In this study,an amino acid analyzer method is established for the simultaneous determination of eight biogenic amines (i.e.,putrescine,histamine,cadaverine,spermidine,octopamine,spermine,tyramine,and phenylethylamine ) in aquatic animal products.The aquatic animal product samples are extracted using a sulfosalicylic acid solution,which is followed by high -speed refrigerated centrifugation,elution with potassium salt buffer,separation using a potassium ionic sulfonic acid based strong acid cation exchange chromatographic column,online derivatization with ninhydrin at 130 ℃,and detection at 570 nm.［Results］ The linear ranges for the eight biogenic amines are 1.0~50.0 mg/L with correlation coefficients (R2) greater than 0.994 7.The limits of detection range from 2.5 to 5.0 mg/kg,and the limits of quantification range from 8.3 to 16.7 mg/kg.The spike recovery rates for the three matrices range from 62.2% to 119.2%.The derivatization conditions and equipment stability are satisfactory,with relative standard deviations (RSD ) of peak areas ranging from 0.43% to 3.89% and RSD of retention time ranging from 0.050% to 0.334%.［Conclusion］ This developed method exhibits excellent stability,sensitivity,recovery,and accuracy.It is a rapid and simple approach for the detection of the eight biogenic amines in aquatic animal products.

Publication Date

4-3-2025

First Page

65

Last Page

71

DOI

10.13652/j.spjx.1003.5788.2024.80588

Recommended Citation

Hongyan, FENG; Yongsheng, XIA; Liangliang, QIAN; Xiaojuan, CHEN; and Jing, ZHOU (2025) "Quantification of eight biogenic amines in aquatic animal products with amino acid analyzer," Food and Machinery: Vol. 41: Iss. 2, Article 8.
DOI: 10.13652/j.spjx.1003.5788.2024.80588
Available at: https://www.ifoodmm.cn/journal/vol41/iss2/8

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